Functional development of the adult ovine mammary gland--insights from gene expression profiling.

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dc.citation.volume16
dc.contributor.authorPaten AM
dc.contributor.authorDuncan EJ
dc.contributor.authorPain SJ
dc.contributor.authorPeterson SW
dc.contributor.authorKenyon PR
dc.contributor.authorBlair HT
dc.contributor.authorDearden PK
dc.date.available2015-10-05
dc.date.available2015-09-22
dc.date.issued2015-10-05
dc.description.abstractBACKGROUND: The mammary gland is a dynamic organ that undergoes dramatic physiological adaptations during the transition from late pregnancy to lactation. Investigation of the molecular basis of mammary development and function will provide fundamental insights into tissue remodelling as well as a better understanding of milk production and mammary disease. This is important to livestock production systems and human health. Here we use RNA-seq to identify differences in gene expression in the ovine mammary gland between late pregnancy and lactation. RESULTS: Between late pregnancy (135 days of gestation ± 2.4 SD) and lactation (15 days post partum ± 1.27 SD) 13 % of genes in the sheep genome were differentially expressed in the ovine mammary gland. In late pregnancy, cell proliferation, beta-oxidation of fatty acids and translation were identified as key biological processes. During lactation, high levels of milk fat synthesis were mirrored by enrichment of genes associated with fatty acid biosynthesis, transport and lipogenesis. Protein processing in the endoplasmic reticulum was enriched during lactation, likely in support of active milk protein synthesis. Hormone and growth factor signalling and activation of signal transduction pathways, including the JAK-STAT and PPAR pathways, were also differently regulated, indicating key roles for these pathways in functional development of the ovine mammary gland. Changes in the expression of epigenetic regulators, particularly chromatin remodellers, indicate a possible role in coordinating the large-scale transcriptional changes that appear to be required to switch mammary processes from growth and development during late pregnancy to synthesis and secretion of milk during lactation. CONCLUSIONS: Coordinated transcriptional regulation of large numbers of genes is required to switch between mammary tissue establishment during late pregnancy, and activation and maintenance of milk production during lactation. Our findings indicate the remarkable plasticity of the mammary gland, and the coordinated regulation of multiple genes and pathways to begin milk production. Genes and pathways identified by the present study may be important for managing milk production and mammary development, and may inform studies of diseases affecting the mammary gland.
dc.description.publication-statusPublished
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000362253000009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=c5bb3b2499afac691c2e3c1a83ef6fef
dc.identifierARTN 748
dc.identifier.citationBMC GENOMICS, 2015, 16
dc.identifier.doi10.1186/s12864-015-1947-9
dc.identifier.elements-id259028
dc.identifier.harvestedMassey_Dark
dc.identifier.issn1471-2164
dc.relation.isPartOfBMC GENOMICS
dc.rights© 2015 Paten et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
dc.subjectSheep
dc.subjectMammary gland
dc.subjectRNA-seq
dc.subjectDevelopment
dc.subjectLactation
dc.subject.anzsrc06 Biological Sciences
dc.subject.anzsrc08 Information and Computing Sciences
dc.subject.anzsrc11 Medical and Health Sciences
dc.titleFunctional development of the adult ovine mammary gland--insights from gene expression profiling.
dc.typeJournal article
pubs.notesNot known
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Sciences
pubs.organisational-group/Massey University/College of Sciences/School of Agriculture & Environment
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