Unravelling the genomic structure of Saccharomyces cerevisiae : a thesis submitted for the degree of Doctor of Philosophy at Massey University, Auckland, New Zealand

Rodley, Christopher David Melchoir

Unravelling the genomic structure of Saccharomyces cerevisiae : a thesis submitted for the degree of Doctor of Philosophy at Massey University, Auckland, New Zealand

dc.contributor.author	Rodley, Christopher David Melchoir
dc.date.accessioned	2011-11-10T23:48:55Z
dc.date.available	2011-11-10T23:48:55Z
dc.date.issued	2011
dc.description	Content removed due to copyright restrictions: Rodley, C.D.M, Bertels, F., Jones, B. & O'Sullivan, J.M. (2009). Global identification of yeast chromosome interactions using Genome conformation capture. Fungal Genetics and Biology. 46(11), 879-886. doi:10.1016/j.fgb.2009.07.006. Also the following computer programmes: Manipulation program (v3) ; Milchreis: Operations on big genomes (version 1.21) ; DNA topography tool (version GerdlsChanges 1.19)
dc.description.abstract	Advances in high-throughput sequencing technology have enabled the sequencing of genomes for many organisms. But the ability to describe the linear arrangement of genetic elements on the chromosomes does not tell us much about how all of these elements work in concert to form and maintain a functional cell. To get some way towards a more holistic understanding of how this is achieved requires the elucidation of three-dimensional genome organisation. The association of chromosomes with each other and other nuclear components plays a critical role in nuclear organisation and genome function. Interactions, which can be structural or functional in nature, form between different parts of the genome. Chromosomal interactions can be broadly divided into two groups, inter- and intra- chromosomal interactions, depending upon whether the interaction forms between different chromosomes or within a single chromosome, respectively. Here I describe a methodology capable of capturing these interactions on a global scale, Genome Conformation Capture (GCC), and reveal the interaction network for Saccharomyces cerevisiae. The inter- and intra- chromosomal interactions detected by GCC are non-random and include contacts between the nuclear chromosomes, 2-micron plasmid, and the mitochondrial genome. These results formed the first global map of chromosomal interactions in a eukaryotic nucleus and demonstrated the highly connected nature of the yeast genome. I subsequently performed GCC on S. cerevisiae cells grown on glucose, galactose, and glycerol lactate to investigate how genome organisation alters depending upon the metabolic regime being employed. I describe the difference in the numbers and types of interactions that form in the three conditions and investigate interactions involving transfer RNAs in detail. Interactions between the mitochondrial and nuclear genomes undergo significant changes depending upon the carbon source on which the yeast is grown. The nuclear and mitochondrial organelles must maintain a communication system in order to respond effectively to environmental conditions. Previous studies have identified mitochondrial DNA inside the nucleus and interacting with the nuclear chromosomes. How this transfer occurs and what the function of the mitochondrial III DNA is once inside the nucleus remains unclear. Here I isolate interactions between the mitochondrial and nuclear genomes and demonstrate dependence upon mitochondrial encoded reverse transcriptase machinery. Furthermore, the nuclear gene transcript level is altered when the interaction frequency between the mitochondrial and nuclear genome is reduced. I conclude that mitochondrial DNA interactions with the nuclear genome are biologically relevant and that the results argue for a role for reverse transcription in inter-organelle DNA mediated communication. The results presented in this thesis have significant implications for our understanding of eukaryotic genome organization.	en_US
dc.identifier.uri	http://hdl.handle.net/10179/2861
dc.language.iso	en	en_US
dc.publisher	Massey University	en_US
dc.rights	The Author	en_US
dc.subject	Saccharomyces cerevisiae	en_US
dc.subject	Genetics	en_US
dc.subject	Genome dynamics	en_US
dc.subject	Genome analysis	en_US
dc.title	Unravelling the genomic structure of Saccharomyces cerevisiae : a thesis submitted for the degree of Doctor of Philosophy at Massey University, Auckland, New Zealand	en_US
dc.type	Thesis	en_US
massey.contributor.author	Rodley, Christopher David Melchoir
thesis.degree.discipline	Genetics
thesis.degree.grantor	Massey University
thesis.degree.level	Doctoral
thesis.degree.level	Doctoral	en
thesis.degree.name	Doctor of Philosophy (Ph.D.)