Role of a local transcription factor in governing cellular carbon/nitrogen homeostasis in Pseudomonas fluorescens

Simple item page
dc.citation.issue6
dc.citation.volume49
dc.contributor.authorNaren N
dc.contributor.authorZhang X-X
dc.coverage.spatialEngland
dc.date.accessioned2024-01-15T20:00:09Z
dc.date.accessioned2024-07-25T06:33:57Z
dc.date.available2021-03-01
dc.date.available2024-01-15T20:00:09Z
dc.date.available2024-07-25T06:33:57Z
dc.date.issued2021-04-06
dc.description.abstractAutoactivation of two-component systems (TCSs) can increase the sensitivity to signals but inherently cause a delayed response. Here, we describe a unique negative feedback mechanism enabling the global NtrB/NtrC regulator to rapidly respond to nitrogen starvation over the course of histidine utilization (hut) in Pseudomonas fluorescens. NtrBC directly activates transcription of hut genes, but overexpression will produce excess ammonium leading to NtrBC inactivation. To prevent this from occurring, the histidine-responsive repressor HutC fine-tunes ntrBC autoactivation: HutC and NtrC bind to the same operator site in the ntrBC promoter. This newly discovered low-affinity binding site shows little sequence similarity with the consensus sequence that HutC recognizes for substrate-specific induction of hut operons. A combination of genetic and transcriptomic analysis indicated that both ntrBC and hut promoter activities cannot be stably maintained in the ΔhutC background when histidine fluctuates at high concentrations. Moreover, the global carbon regulator CbrA/CbrB is involved in directly activating hut transcription while de-repressing hut translation via the CbrAB-CrcYZ-Crc/Hfq regulatory cascade. Together, our data reveal that the local transcription factor HutC plays a crucial role in governing NtrBC to maintain carbon/nitrogen homeostasis through the complex interactions between two TCSs (NtrBC and CbrAB) at the hut promoter.
dc.description.confidentialfalse
dc.format.pagination3204-3216
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/33675669
dc.identifier.citationNaren N, Zhang X-X. (2021). Role of a local transcription factor in governing cellular carbon/nitrogen homeostasis in Pseudomonas fluorescens.. Nucleic Acids Res. 49. 6. (pp. 3204-3216).
dc.identifier.doi10.1093/nar/gkab091
dc.identifier.eissn1362-4962
dc.identifier.elements-typejournal-article
dc.identifier.issn0305-1048
dc.identifier.pii6155939
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/70434
dc.languageeng
dc.publisherOxford University Press on behalf of Nucleic Acids Research
dc.publisher.urihttps://academic.oup.com/nar/article/49/6/3204/6155939
dc.relation.isPartOfNucleic Acids Res
dc.rights(c) 2021 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectBacterial Proteins
dc.subjectBinding Sites
dc.subjectCarbon
dc.subjectFeedback, Physiological
dc.subjectGene Expression Regulation, Bacterial
dc.subjectHistidine
dc.subjectHomeostasis
dc.subjectNitrogen
dc.subjectPromoter Regions, Genetic
dc.subjectPseudomonas fluorescens
dc.subjectRepressor Proteins
dc.subjectTranscription Factors
dc.subjectTranscriptional Activation
dc.titleRole of a local transcription factor in governing cellular carbon/nitrogen homeostasis in Pseudomonas fluorescens
dc.typeJournal article
pubs.elements-id440938
pubs.organisational-groupOther
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