Sequential breakdown of the Cf-9 leaf mould resistance locus in tomato by Fulvia fulva.

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dc.citation.volumeEarly View
dc.contributor.authorde la Rosa S
dc.contributor.authorSchol CR
dc.contributor.authorRamos Peregrina Á
dc.contributor.authorWinter DJ
dc.contributor.authorHilgers AM
dc.contributor.authorMaeda K
dc.contributor.authorIida Y
dc.contributor.authorTarallo M
dc.contributor.authorJia R
dc.contributor.authorBeenen HG
dc.contributor.authorRocafort M
dc.contributor.authorde Wit PJGM
dc.contributor.authorBowen JK
dc.contributor.authorBradshaw RE
dc.contributor.authorJoosten MHAJ
dc.contributor.authorBai Y
dc.contributor.authorMesarich CH
dc.coverage.spatialEngland
dc.date.accessioned2024-10-03T01:31:13Z
dc.date.available2024-10-03T01:31:13Z
dc.date.issued2024-06-24
dc.description.abstractLeaf mould, caused by Fulvia fulva, is a devastating disease of tomato plants. In many commercial tomato cultivars, resistance to this disease is governed by the Cf-9 locus, which encodes five paralogous receptor-like proteins. Two of these proteins confer resistance: Cf-9C recognises the previously identified F. fulva effector Avr9 and provides resistance during all plant growth stages, while Cf-9B recognises the yet-unidentified F. fulva effector Avr9B and provides mature plant resistance only. In recent years, F. fulva strains have emerged that can overcome the Cf-9 locus, with Cf-9C circumvented through Avr9 deletion. To understand how Cf-9B is circumvented, we set out to identify Avr9B. Comparative genomics, transient expression assays and gene complementation experiments were used to identify Avr9B, while gene sequencing was used to assess Avr9B allelic variation across a world-wide strain collection. A strict correlation between Avr9 deletion and resistance-breaking mutations in Avr9B was observed in strains recently collected from Cf-9 cultivars, whereas Avr9 deletion but no mutations in Avr9B were observed in older strains. This research showcases how F. fulva has evolved to sequentially break down the Cf-9 locus and stresses the urgent need for commercial tomato cultivars that carry novel, stacked resistance genes active against this pathogen.
dc.description.confidentialfalse
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/38922927
dc.identifier.citationde la Rosa S, Schol CR, Ramos Peregrina Á, Winter DJ, Hilgers AM, Maeda K, Iida Y, Tarallo M, Jia R, Beenen HG, Rocafort M, de Wit PJGM, Bowen JK, Bradshaw RE, Joosten MHAJ, Bai Y, Mesarich CH. (2024). Sequential breakdown of the Cf-9 leaf mould resistance locus in tomato by Fulvia fulva.. New Phytol. Early View.
dc.identifier.doi10.1111/nph.19925
dc.identifier.eissn1469-8137
dc.identifier.elements-typejournal-article
dc.identifier.issn0028-646X
dc.identifier.number19925
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71580
dc.languageeng
dc.publisherJohn Wiley and Sons Ltd on behalf of New Phytologist Foundation
dc.publisher.urihttps://nph.onlinelibrary.wiley.com/doi/10.1111/nph.19925
dc.relation.isPartOfNew Phytol
dc.rights(c) 2024 The Author/s
dc.rightsCC BY-NC-ND 4.0
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAvr9 and Avr9B avirulence effector genes
dc.subjectCf‐9 locus
dc.subjectCf‐9C and Cf‐9B resistance genes
dc.subjectFulvia fulva (Cladosporium fulvum)
dc.subjectSolanum lycopersicum (tomato)
dc.subjectfungus
dc.subjectsequential resistance breakdown
dc.subjecttomato leaf mould disease
dc.titleSequential breakdown of the Cf-9 leaf mould resistance locus in tomato by Fulvia fulva.
dc.typeJournal article
pubs.elements-id489490
pubs.organisational-groupCollege of Health
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