Archaeal pseudomurein and bacterial murein cell wall biosynthesis share a common evolutionary ancestry

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dc.citation.volume2
dc.contributor.authorSubedi B
dc.contributor.authorMartin WF
dc.contributor.authorCarbone V
dc.contributor.authorDuin EC
dc.contributor.authorCronin B
dc.contributor.authorSauter J
dc.contributor.authorSchofield LR
dc.contributor.authorSutherland-Smith A
dc.contributor.authorRonimus RS
dc.date.available2021-08-24
dc.date.issued2021-08-24
dc.description.abstractBacteria near-universally contain a cell wall sacculus of murein (peptidoglycan), the synthesis of which has been intensively studied for over 50 years. In striking contrast, archaeal species possess a variety of other cell wall types, none of them closely resembling murein. Interestingly though, one type of archaeal cell wall termed pseudomurein found in the methanogen orders Methanobacteriales and Methanopyrales is a structural analogue of murein in that it contains a glycan backbone that is cross-linked by a L-amino acid peptide. Here, we present taxonomic distribution, gene cluster and phylogenetic analyses that confirm orthologues of 13 bacterial murein biosynthesis enzymes in pseudomurein-containing methanogens, most of which are distantly related to their bacterial counterparts. We also present the first structure of an archaeal pseudomurein peptide ligase from Methanothermus fervidus DSM1088 (Mfer336) to a resolution of 2.5 A and show that it possesses a similar overall tertiary three domain structure to bacterial MurC and MurD type murein peptide ligases. Taken together the data strongly indicate that murein and pseudomurein biosynthetic pathways share a common evolutionary history.
dc.description.confidentialfalse
dc.format.extent1 - 13
dc.identifierhttps://academic.oup.com/femsmicrobes/article/doi/10.1093/femsmc/xtab012/6356954
dc.identifierxtab012
dc.identifier.citationFEMS Microbes, 2021, 2 pp. 1 - 13
dc.identifier.doi10.1093/femsmc/xtab012
dc.identifier.elements-id449236
dc.identifier.harvestedMassey_Dark
dc.publisherFEMS Oxford University Press
dc.publisher.urihttps://academic.oup.com/femsmicrobes/article/doi/10.1093/femsmc/xtab012/6356954
dc.relation.isPartOfFEMS Microbes
dc.relation.urihttps://academic.oup.com/femsmicrobes/article/doi/10.1093/femsmc/xtab012/6356954
dc.rights(c) The Author(s) 2021. Published by Oxford University Press on behalf of FEMS. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.titleArchaeal pseudomurein and bacterial murein cell wall biosynthesis share a common evolutionary ancestry
dc.typeJournal article
pubs.notesNot known
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Sciences
pubs.organisational-group/Massey University/College of Sciences/School of Natural Sciences
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