Population structure and pathogen interaction of Escherichia coli in freshwater: Implications of land-use for water quality and public health in Aotearoa New Zealand.

Simple item page
dc.citation.issue4
dc.citation.volume16
dc.contributor.authorCookson AL
dc.contributor.authorDevane M
dc.contributor.authorMarshall JC
dc.contributor.authorMoinet M
dc.contributor.authorGardner A
dc.contributor.authorCollis RM
dc.contributor.authorRogers L
dc.contributor.authorBiggs PJ
dc.contributor.authorPita AB
dc.contributor.authorCornelius AJ
dc.contributor.authorHaysom I
dc.contributor.authorHayman DTS
dc.contributor.authorGilpin BJ
dc.contributor.authorLeonard M
dc.coverage.spatialUnited States
dc.date.accessioned2024-08-14T20:43:21Z
dc.date.available2024-08-14T20:43:21Z
dc.date.issued2024-08-02
dc.description.abstractFreshwater samples (n = 199) were obtained from 41 sites with contrasting land-uses (avian, low impact, dairy, urban, sheep and beef, and mixed sheep, beef and dairy) and the E. coli phylotype of 3980 isolates (20 per water sample enrichment) was determined. Eight phylotypes were identified with B1 (48.04%), B2 (14.87%) and A (14.79%) the most abundant. Escherichia marmotae (n = 22), and Escherichia ruysiae (n = 1), were rare (0.68%) suggesting that these environmental strains are unlikely to confound water quality assessments. Phylotypes A and B1 were overrepresented in dairy and urban sites (p < 0.0001), whilst B2 were overrepresented in low impact sites (p < 0.0001). Pathogens ((Salmonella, Campylobacter, Cryptosporidium or Giardia) and the presence of diarrhoeagenic E. coli-associated genes (stx and eae) were detected in 89.9% (179/199) samples, including 80.5% (33/41) of samples with putative non-recent faecal inputs. Quantitative PCR to detect microbial source tracking targets from human, ruminant and avian contamination were concordant with land-use type and E. coli phylotype abundance. This study demonstrated that a potential recreational health risk remains where pathogens occurred in water samples with low E. coli concentration, potential non-recent faecal sources, low impact sites and where human, ruminant and avian faecal sources were absent.
dc.description.confidentialfalse
dc.edition.editionAugust 2024
dc.format.paginatione13319-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/39096033
dc.identifier.citationCookson AL, Devane M, Marshall JC, Moinet M, Gardner A, Collis RM, Rogers L, Biggs PJ, Pita AB, Cornelius AJ, Haysom I, Hayman DTS, Gilpin BJ, Leonard M. (2024). Population structure and pathogen interaction of Escherichia coli in freshwater: Implications of land-use for water quality and public health in Aotearoa New Zealand.. Environ Microbiol Rep. 16. 4. (pp. e13319-).
dc.identifier.doi10.1111/1758-2229.13319
dc.identifier.eissn1758-2229
dc.identifier.elements-typejournal-article
dc.identifier.issn1758-2229
dc.identifier.numbere13319
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71297
dc.languageeng
dc.publisherJohn Wiley & Sons, Inc.
dc.publisher.urihttps://enviromicro-journals.onlinelibrary.wiley.com/doi/10.1111/1758-2229.13319
dc.relation.isPartOfEnviron Microbiol Rep
dc.rights(c) 2024 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectNew Zealand
dc.subjectEscherichia coli
dc.subjectFresh Water
dc.subjectWater Quality
dc.subjectPublic Health
dc.subjectAnimals
dc.subjectHumans
dc.subjectWater Microbiology
dc.subjectPhylogeny
dc.subjectFeces
dc.subjectCryptosporidium
dc.subjectGiardia
dc.titlePopulation structure and pathogen interaction of Escherichia coli in freshwater: Implications of land-use for water quality and public health in Aotearoa New Zealand.
dc.typeJournal article
pubs.elements-id490944
pubs.organisational-groupOther
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Published version.pdf
Size:
3.87 MB
Format:
Adobe Portable Document Format
Description:
490944 PDF.pdf
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
9.22 KB
Format:
Plain Text
Description:
Download
Collections
Journal Articles