Microtiter Screening Reveals Oxygen-Dependent Antimicrobial Activity of Natural Products Against Mastitis-Causing Bacteria

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dc.citation.issueAUG
dc.citation.volume10
dc.contributor.authorFerguson SA
dc.contributor.authorMenorca A
dc.contributor.authorVan Zuylen EM
dc.contributor.authorCheung C-Y
dc.contributor.authorMcConnell MA
dc.contributor.authorRennison D
dc.contributor.authorBrimble MA
dc.contributor.authorBodle K
dc.contributor.authorMcDougall S
dc.contributor.authorCook GM
dc.contributor.authorHeikal A
dc.contributor.editorDebnath A
dc.coverage.spatialSwitzerland
dc.date.accessioned2024-02-07T00:28:48Z
dc.date.accessioned2024-07-25T06:45:41Z
dc.date.available2019-08-28
dc.date.available2024-02-07T00:28:48Z
dc.date.available2024-07-25T06:45:41Z
dc.date.issued2019-08-28
dc.description.abstractIn this study we investigated the influence of oxygen availability on a phenotypic microtiter screen to identify new, natural product inhibitors of growth for the bovine mastitis-causing microorganisms; Streptococcus uberis, Staphylococcus aureus, and Escherichia coli. Mastitis is a common disease in dairy cattle worldwide and is a major cause of reduced milk yield and antibiotic usage in dairy herds. Prevention of bovine mastitis commonly relies on the application of teat disinfectants that contain either iodine or chlorhexidine. These compounds are used extensively in human clinical settings and increased tolerance to chlorhexidine has been reported in both Gram-positive and Gram-negative microorganisms. As such new, non-human use alternatives are required for the agricultural industry. Our screening was conducted under normoxic (20% oxygen) and hypoxic (<1% oxygen) conditions to mimic the conditions on teat skin and within the mammary gland respectively, against two natural compound libraries. No compounds inhibited E. coli under either oxygen condition. Against the Gram-positive microorganisms, 12 inhibitory compounds were identified under normoxic conditions, and 10 under hypoxic conditions. Data revealed a clear oxygen-dependency amongst compounds inhibiting growth, with only partial overlap between oxygen conditions. The oxygen-dependent inhibitory activity of a naturally occurring quinone, β-lapachone, against S. uberis was subsequently investigated and we demonstrated that this compound is only active under normoxic conditions with a minimum inhibitory concentration and minimum bactericidal concentration of 32 μM and kills via a reactive oxygen species-dependent mechanism as has been demonstrated in other microorganisms. These results demonstrate the importance of considering oxygen-availability in high-throughput inhibitor discovery.
dc.description.confidentialfalse
dc.format.pagination1995-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/31555233
dc.identifier.citationFerguson SA, Menorca A, Van Zuylen EM, Cheung C-Y, McConnell MA, Rennison D, Brimble MA, Bodle K, McDougall S, Cook GM, Heikal A. (2019). Microtiter Screening Reveals Oxygen-Dependent Antimicrobial Activity of Natural Products Against Mastitis-Causing Bacteria.. Front Microbiol. 10. AUG. (pp. 1995-).
dc.identifier.doi10.3389/fmicb.2019.01995
dc.identifier.eissn1664-302X
dc.identifier.elements-typejournal-article
dc.identifier.issn1664-302X
dc.identifier.numberARTN 1995
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/70816
dc.languageeng
dc.publisherFrontiers Media S.A.
dc.publisher.urihttps://www.frontiersin.org/articles/10.3389/fmicb.2019.01995/full
dc.relation.isPartOfFront Microbiol
dc.rights(c) The author/sen
dc.rights.licenseCC BY 4.0en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectStaphylococcus aureus
dc.subjectStreptococcus uberis
dc.subjectantimicrobial
dc.subjectmastitis
dc.subjectnatural product inhibitors
dc.subjectoxygen-dependent
dc.subjectβ-lapachone
dc.titleMicrotiter Screening Reveals Oxygen-Dependent Antimicrobial Activity of Natural Products Against Mastitis-Causing Bacteria
dc.typeJournal article
pubs.elements-id445630
pubs.organisational-groupOther
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