Functional analysis and subcellular localization of two geranylgeranyl diphosphate synthases from Penicillium paxilli

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dc.citation.issue3
dc.citation.volume282
dc.contributor.authorSaikia S
dc.contributor.authorScott B
dc.date.available2009-09-01
dc.date.available2009-05-28
dc.date.issued2009
dc.description.abstractThe filamentous fungus Penicillium paxilli contains two distinct geranylgeranyl diphosphate (GGPP) synthases, GgsA and GgsB (PaxG). PaxG and its homologues in Neotyphodium lolii and Fusarium fujikuroi are associated with diterpene secondary metabolite gene clusters. The genomes of other filamentous fungi including Aspergillus fumigatus, Aspergillus nidulans, Aspergillus niger, Aspergillus oryzae and Fusarium graminearum also contain two or more copies of GGPP synthase genes, although the diterpene metabolite capability of these fungi is not known. The objective of this study was to understand the biological significance of the presence of two copies of GGPP synthases in P. paxilli by investigating their subcellular localization. Using a carotenoid complementation assay and gene deletion analysis, we show that P. paxilli GgsA and PaxG have GGPP synthase activities and that paxG is required for paxilline biosynthesis, respectively. In the DeltapaxG mutant background ggsA was unable to complement paxilline biosynthesis. A GgsA-EGFP fusion protein was localized to punctuate organelles and the EGFP-GRV fusion protein, containing the C-terminus tripeptide GRV of PaxG, was localized to peroxisomes. A truncated PaxG mutant lacking the C-terminus tripeptide GRV was unable to complement a DeltapaxG mutant demonstrating that the tripeptide is functionally important for paxilline biosynthesis.
dc.description.publication-statusPublished
dc.format.extent257 - 271 (15)
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000269206500004&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=c5bb3b2499afac691c2e3c1a83ef6fef
dc.identifier.citationMOLECULAR GENETICS AND GENOMICS, 2009, 282 (3), pp. 257 - 271 (15)
dc.identifier.doi10.1007/s00438-009-0463-5
dc.identifier.elements-id34082
dc.identifier.harvestedMassey_Dark
dc.identifier.issn1617-4615
dc.identifier.urihttps://hdl.handle.net/10179/13237
dc.languageEnglish
dc.publisherSpringer
dc.relation.isPartOfMOLECULAR GENETICS AND GENOMICS
dc.relation.urihttps://link.springer.com/article/10.1007/s00438-009-0463-5
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectBiochemistry & Molecular Biology
dc.subjectGenetics & Heredity
dc.subjectBIOCHEMISTRY & MOLECULAR BIOLOGY
dc.subjectGENETICS & HEREDITY
dc.subjectGeranylgeranyl diphosphate synthase
dc.subjectSubcellular localization
dc.subjectPeroxisome
dc.subjectIndole-diterpene
dc.subjectSecondary metabolism
dc.subjectPenicillium paxilli
dc.subjectJAPANESE PEAR PATHOTYPE
dc.subjectDITERPENE GENE-CLUSTER
dc.subjectASPERGILLUS-NIDULANS
dc.subjectINDOLE-DITERPENE
dc.subjectBETA-OXIDATION
dc.subjectSACCHAROMYCES-CEREVISIAE
dc.subjectPYROPHOSPHATE SYNTHASE
dc.subjectFRACTIONATED COLONIES
dc.subjectBIOSYNTHETIC-PATHWAY
dc.subjectALTERNARIA-ALTERNATA
dc.subject.anzsrc0604 Genetics
dc.subject.anzsrc0607 Plant Biology
dc.titleFunctional analysis and subcellular localization of two geranylgeranyl diphosphate synthases from Penicillium paxilli
dc.typeJournal article
pubs.notesNot known
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Sciences
pubs.organisational-group/Massey University/College of Sciences/School of Fundamental Sciences
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