In Vitro Effects of Doxycycline on Replication of Feline Coronavirus

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dc.citation.issue3
dc.citation.volume10
dc.contributor.authorGhosh S
dc.contributor.authorDunowska M
dc.date.available2021-03
dc.date.available2021-03-02
dc.date.issued2021-03-07
dc.description.abstractFeline infectious peritonitis (FIP) is a sporadic fatal disease of cats caused by a virulent variant of feline coronavirus (FCoV), referred to as FIP virus (FIPV). Treatment options are limited, and most of the affected cats die or are euthanized. Anecdotally, doxycycline has been used to treat FIP-affected cats, but there are currently no data to support or discourage such treatment. The aim of this study was to establish whether doxycycline inhibits replication of FIPV in vitro. The virus was cultured in Crandell-Rees feline kidney cells with various concentrations of doxycycline (0 to 50 µg/mL). The level of FIPV in cultures was determined by virus titration and FCoV-specific reverse-transcription quantitative PCR. Cell viability was also monitored. There was no difference in the level of infectious virus or viral RNA between doxycycline-treated and untreated cultures at 3, 12- and 18-hours post-infection. However, at 24 h, the growth of FIPV was inhibited by approximately two logs in cultures with >10 µg/mL doxycycline. This inhibition was dose-dependent, with inhibitory concentration 50% (IC50) 4.1 µg/mL and IC90 5.4 µg/mL. Our data suggest that doxycycline has some inhibitory effect on FIPV replication in vitro, which supports future clinical trials of its use for the treatment of FIP-affected cats.
dc.description.publication-statusPublished
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000633958600001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=c5bb3b2499afac691c2e3c1a83ef6fef
dc.identifierARTN 312
dc.identifier.citationPATHOGENS, 2021, 10 (3)
dc.identifier.doi10.3390/pathogens10030312
dc.identifier.eissn2076-0817
dc.identifier.elements-id441758
dc.identifier.harvestedMassey_Dark
dc.publisherMDPI AG
dc.relation.isPartOfPATHOGENS
dc.rightsThe Author(s) CC BY 4.0
dc.subjectfeline infectious peritonitis (FIP)
dc.subjectfeline coronavirus
dc.subjectdoxycycline
dc.subjectantiviral
dc.subject.anzsrc1107 Immunology
dc.subject.anzsrc1108 Medical Microbiology
dc.titleIn Vitro Effects of Doxycycline on Replication of Feline Coronavirus
dc.typeJournal article
pubs.notesNot known
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Sciences
pubs.organisational-group/Massey University/College of Sciences/School of Veterinary Science
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