Novel hyperthermoacidic archaeal enzymes for removal of thermophilic biofilms from stainless steel

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dc.citation.issue6
dc.citation.volume134
dc.contributor.authorNam Y
dc.contributor.authorBarnebey A
dc.contributor.authorKim HK
dc.contributor.authorYannone SM
dc.contributor.authorFlint S
dc.coverage.spatialEngland
dc.date.accessioned2024-10-02T00:56:31Z
dc.date.available2024-10-02T00:56:31Z
dc.date.issued2023-06
dc.description.abstractAIMS: To test the efficacy of novel hot/acid hyperthermoacidic enzyme treatments on the removal of thermophilic spore-forming biofilms from stainless steel surfaces. METHODS AND RESULTS: The present study measured the efficacy of hyperthermoacidic enzymes (protease, amylase, and endoglucanase) that are optimally active at low pH (≈3.0) and high temperatures (≈80°C) at removing thermophilic bacilli biofilms from stainless steel (SS) surfaces. Plate counts, spore counts, impedance microbiology, as well as epifluorescence microscopy, and scanning electron microscopy (SEM) were used to evaluate the cleaning and sanitation of biofilms grown in a continuous flow biofilm reactor. Previously unavailable hyperthermoacidic amylase, protease, and the combination of amylase and protease were tested on Anoxybacillus flavithermus and Bacillus licheniformis, and endoglucanase was tested on Geobacillus stearothermophilus. In all cases, the heated acidic enzymatic treatments significantly reduced biofilm cells and their sheltering extracellular polymeric substances (EPS). CONCLUSIONS: Hyperthermoacidic enzymes and the associated heated acid conditions are effective at removing biofilms of thermophilic bacteria from SS surfaces that contaminate dairy plants.
dc.description.confidentialfalse
dc.edition.editionJune 2023
dc.format.paginationlxad106-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/37218716
dc.identifier.citationNam Y, Barnebey A, Kim HK, Yannone SM, Flint S. (2023). Novel hyperthermoacidic archaeal enzymes for removal of thermophilic biofilms from stainless steel.. J Appl Microbiol. 134. 6. (pp. lxad106-).
dc.identifier.doi10.1093/jambio/lxad106
dc.identifier.eissn1365-2672
dc.identifier.elements-typejournal-article
dc.identifier.issn1364-5072
dc.identifier.numberlxad106
dc.identifier.pii7176065
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71563
dc.languageeng
dc.publisherOxford University Press on behalf of Applied Microbiology International
dc.publisher.urihttps://academic.oup.com/jambio/article/134/6/lxad106/7176065
dc.relation.isPartOfJ Appl Microbiol
dc.rights(c) 2023 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAnoxybacillus flavithermus
dc.subjectBacillus licheniforms
dc.subjectGeobacillus stearothermophilus
dc.subjectHTA-enzymes
dc.subjectamylase
dc.subjectarchaea
dc.subjectcleaning
dc.subjectdairy
dc.subjectendoglucanase
dc.subjectprotease
dc.subjectspores
dc.subjectAnimals
dc.subjectStainless Steel
dc.subjectMilk
dc.subjectArchaea
dc.subjectCellulase
dc.subjectBiofilms
dc.subjectPeptide Hydrolases
dc.titleNovel hyperthermoacidic archaeal enzymes for removal of thermophilic biofilms from stainless steel
dc.typeJournal article
pubs.elements-id461874
pubs.organisational-groupOther
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