CryoEM structure of the outer membrane secretin channel pIV from the f1 filamentous bacteriophage

Simple item page
dc.citation.volume12
dc.contributor.authorConners R
dc.contributor.authorMcLaren M
dc.contributor.authorŁapińska U
dc.contributor.authorSanders K
dc.contributor.authorStone MRL
dc.contributor.authorBlaskovich MAT
dc.contributor.authorPagliara S
dc.contributor.authorDaum B
dc.contributor.authorRakonjac J
dc.contributor.authorGold VAM
dc.date.accessioned2024-01-08T00:36:31Z
dc.date.accessioned2024-07-25T06:43:29Z
dc.date.available2021-11-02
dc.date.available2024-01-08T00:36:31Z
dc.date.available2024-07-25T06:43:29Z
dc.date.issued2021-11-02
dc.description.abstractThe Ff family of filamentous bacteriophages infect gram-negative bacteria, but do not cause lysis of their host cell. Instead, new virions are extruded via the phage-encoded pIV protein, which has homology with bacterial secretins. Here, we determine the structure of pIV from the f1 filamentous bacteriophage at 2.7 Å resolution by cryo-electron microscopy, the first near-atomic structure of a phage secretin. Fifteen f1 pIV subunits assemble to form a gated channel in the bacterial outer membrane, with associated soluble domains projecting into the periplasm. We model channel opening and propose a mechanism for phage egress. By single-cell microfluidics experiments, we demonstrate the potential for secretins such as pIV to be used as adjuvants to increase the uptake and efficacy of antibiotics in bacteria. Finally, we compare the f1 pIV structure to its homologues to reveal similarities and differences between phage and bacterial secretins.
dc.description.confidentialfalse
dc.identifier.citationConners R, McLaren M, Łapińska U, Sanders K, Stone R, Blaskovich M, Pagliara S, Daum B, Rakonjac J, Gold V. (2021). CryoEM structure of the outer membrane secretin channel pIV from the f1 filamentous bacteriophage. Nature Communications. 12.
dc.identifier.doi10.1101/2021.07.20.453082
dc.identifier.eissn2041-1723
dc.identifier.elements-typejournal-article
dc.identifier.number6316
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/70750
dc.languageEnglish
dc.publisherSpringer Nature Limited
dc.publisher.urihttps://www.nature.com/articles/s41467-021-26610-3
dc.relation.isPartOfNature Communications
dc.rightsCC BY 4.0
dc.rights(c) 2021 The Author/s
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectCryoelectron microscopy
dc.subjectMembrane proteins
dc.subjectPermeation and transport
dc.subjectPhage biology
dc.titleCryoEM structure of the outer membrane secretin channel pIV from the f1 filamentous bacteriophage
dc.typeJournal article
pubs.elements-id453145
pubs.organisational-groupOther
Files
Original bundle
Now showing 1 - 5 of 5
Thumbnail Image
Name:
Published version
Size:
8.3 MB
Format:
Adobe Portable Document Format
Description:
Download
Thumbnail Image
Name:
Evidence 1
Size:
329.92 KB
Format:
Adobe Portable Document Format
Description:
Download
Thumbnail Image
Name:
Evidence 2
Size:
329.92 KB
Format:
Adobe Portable Document Format
Description:
Download
Thumbnail Image
Name:
Evidence 3
Size:
329.92 KB
Format:
Adobe Portable Document Format
Description:
Download
Thumbnail Image
Name:
Evidence 4
Size:
329.92 KB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Journal Articles