Modeling multiple duplex DNA attachments in a force-extension experiment

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dc.citation.issue1
dc.citation.volume2
dc.contributor.authorRaudsepp A
dc.contributor.authorWilliams MAK
dc.contributor.authorJameson GB
dc.contributor.editorEnderlein J
dc.coverage.spatialUnited States
dc.date.accessioned2023-11-06T20:03:15Z
dc.date.accessioned2023-11-07T22:21:42Z
dc.date.available2022-02-02
dc.date.available2023-11-06T20:03:15Z
dc.date.available2023-11-07T22:21:42Z
dc.date.issued2022-03-09
dc.description.abstractOptical tweezers-based DNA stretching often relies on tethering a single end-activated DNA molecule between optically manipulated end-binding beads. Measurement success can depend on DNA concentration. At lower DNA concentrations tethering is less common, and many trials may be required to observe a single-molecule stretch. At higher DNA concentrations tethering is more common; however, the resulting force-extensions observed are more complex and may vary from measurement to measurement. Typically these more complex results are attributed to the formation of multiple tethers between the beads; however, to date there does not appear to have been a critical examination of this hypothesis or the potential usefulness of such data. Here we examine stretches at a higher DNA concentration and use analysis and simulation to show how the more complex force-extensions observed can be understood in terms of multiple DNA attachments.
dc.description.confidentialfalse
dc.format.pagination100045-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/36425083
dc.identifier.citationRaudsepp A, Williams MAK, Jameson GB. (2022). Modeling multiple duplex DNA attachments in a force-extension experiment.. Biophys Rep (N Y). 2. 1. (pp. 100045-).
dc.identifier.doi10.1016/j.bpr.2022.100045
dc.identifier.eissn2667-0747
dc.identifier.elements-typejournal-article
dc.identifier.issn2667-0747
dc.identifier.number100045
dc.identifier.piiS2667-0747(22)00002-7
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/69068
dc.languageeng
dc.publisherCell Press
dc.publisher.urihttps://www.sciencedirect.com/science/article/pii/S2667074722000027
dc.relation.isPartOfBiophys Rep (N Y)
dc.rights(c) 2022 The Author/s
dc.rightsCC BY-NC-ND 4.0
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleModeling multiple duplex DNA attachments in a force-extension experiment
dc.typeJournal article
pubs.elements-id454949
pubs.organisational-groupOther
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