A large chromosomal inversion affects antimicrobial sensitivity of Escherichia coli to sodium deoxycholate

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dc.citation.issue8
dc.citation.volume168
dc.contributor.authorLe VVH
dc.contributor.authorLeón-Quezada RI
dc.contributor.authorBiggs PJ
dc.contributor.authorRakonjac J
dc.coverage.spatialEngland
dc.date.accessioned2024-01-08T22:55:10Z
dc.date.accessioned2024-07-25T06:41:34Z
dc.date.available2022-08-12
dc.date.available2024-01-08T22:55:10Z
dc.date.available2024-07-25T06:41:34Z
dc.date.issued2022-08-12
dc.description.abstractResistance to antimicrobials is normally caused by mutations in the drug targets or genes involved in antimicrobial activation or expulsion. Here we show that an Escherichia coli strain, named DOC14, selected for increased resistance to the bile salt sodium deoxycholate, has no mutations in any ORF, but instead has a 2.1 Mb chromosomal inversion. The breakpoints of the inversion are two inverted copies of an IS5 element. Besides lowering deoxycholate susceptibility, the IS5-mediated chromosomal inversion in the DOC14 mutant was found to increase bacterial survival upon exposure to ampicillin and vancomycin, and sensitize the cell to ciprofloxacin and meropenem, but does not affect bacterial growth or cell morphology in a rich medium in the absence of antibacterial molecules. Overall, our findings support the notion that a large chromosomal inversion can benefit bacterial cells under certain conditions, contributing to genetic variability available for selection during evolution. The DOC14 mutant paired with its isogenic parental strain form a useful model as bacterial ancestors in evolution experiments to study how a large chromosomal inversion influences the evolutionary trajectory in response to various environmental stressors.
dc.description.confidentialfalse
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/35960647
dc.identifier.citationLe VVH, León-Quezada RI, Biggs PJ, Rakonjac J. (2022). A large chromosomal inversion affects antimicrobial sensitivity of Escherichia coli to sodium deoxycholate.. Microbiology (Reading). 168. 8.
dc.identifier.doi10.1099/mic.0.001232
dc.identifier.eissn1465-2080
dc.identifier.elements-typejournal-article
dc.identifier.issn1350-0872
dc.identifier.number001232
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/70691
dc.languageeng
dc.publisherMicrobiology Society
dc.publisher.urihttps://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.001232#html_fulltext
dc.relation.isPartOfMicrobiology (Reading)
dc.rights(c) 2021 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectIS5 element
dc.subjectantimicrobial susceptibility
dc.subjectchromosomal inversion
dc.subjecthybrid genome assembly
dc.subjectsodium deoxycholate
dc.subjectAnti-Bacterial Agents
dc.subjectChromosome Inversion
dc.subjectDeoxycholic Acid
dc.subjectDrug Resistance, Bacterial
dc.subjectEscherichia coli
dc.subjectEscherichia coli Infections
dc.subjectHumans
dc.subjectMicrobial Sensitivity Tests
dc.titleA large chromosomal inversion affects antimicrobial sensitivity of Escherichia coli to sodium deoxycholate
dc.typeJournal article
pubs.elements-id455375
pubs.organisational-groupOther
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