Co-Selection of Bacterial Metal and Antibiotic Resistance in Soil Laboratory Microcosms.

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dc.citation.issue4
dc.citation.volume12
dc.contributor.authorHeydari A
dc.contributor.authorKim ND
dc.contributor.authorBiggs PJ
dc.contributor.authorHorswell J
dc.contributor.authorGielen GJHP
dc.contributor.authorSiggins A
dc.contributor.authorTaylor MD
dc.contributor.authorBromhead C
dc.contributor.authorPalmer BR
dc.coverage.spatialSwitzerland
dc.date.accessioned2023-05-23T21:54:58Z
dc.date.available2023-04-18
dc.date.available2023-04-15
dc.date.available2023-05-23T21:54:58Z
dc.date.issued2023-04-18
dc.descriptionCopyright: © 2023 by the authors.
dc.description.abstractAccumulation of heavy metals (HMs) in agricultural soil following the application of superphosphate fertilisers seems to induce resistance of soil bacteria to HMs and appears to co-select for resistance to antibiotics (Ab). This study aimed to investigate the selection of co-resistance of soil bacteria to HMs and Ab in uncontaminated soil incubated for 6 weeks at 25 °C in laboratory microcosms spiked with ranges of concentrations of cadmium (Cd), zinc (Zn) and mercury (Hg). Co-selection of HM and Ab resistance was assessed using plate culture on media with a range of HM and Ab concentrations, and pollution-induced community tolerance (PICT) assays. Bacterial diversity was profiled via terminal restriction fragment length polymorphism (TRFLP) assay and 16S rDNA sequencing of genomic DNA isolated from selected microcosms. Based on sequence data, the microbial communities exposed to HMs were found to differ significantly compared to control microcosms with no added HM across a range of taxonomic levels.
dc.description.publication-statusPublished online
dc.identifierhttps://www.ncbi.nlm.nih.gov/pubmed/37107134
dc.identifierantibiotics12040772
dc.identifier.citationAntibiotics (Basel), 2023, 12 (4)
dc.identifier.doi10.3390/antibiotics12040772
dc.identifier.elements-id461289
dc.identifier.harvestedMassey_Dark
dc.identifier.issn2079-6382
dc.languageeng
dc.relation.isPartOfAntibiotics (Basel)
dc.relation.urihttps://www.mdpi.com/2079-6382/12/4/772
dc.rightsCC BY
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectPICT
dc.subjectTRFLP
dc.subjectantibiotic resistance
dc.subjectbacteria
dc.subjectco-selection
dc.subjectheavy metal resistance
dc.subjectsoil
dc.subjecttrace elements
dc.titleCo-Selection of Bacterial Metal and Antibiotic Resistance in Soil Laboratory Microcosms.
dc.typeJournal article
pubs.notesNot known
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Health
pubs.organisational-group/Massey University/College of Health/School of Health Science
pubs.organisational-group/Massey University/College of Sciences
pubs.organisational-group/Massey University/College of Sciences/School of Natural Sciences
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