A period of 10 weeks of increased protein consumption does not alter faecal microbiota or volatile metabolites in healthy older men: a randomised controlled trial

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dc.citation.volume9
dc.contributor.authorMitchell SM
dc.contributor.authorMcKenzie EJ
dc.contributor.authorMitchell CJ
dc.contributor.authorMilan AM
dc.contributor.authorZeng N
dc.contributor.authorD'Souza RF
dc.contributor.authorRamzan F
dc.contributor.authorSharma P
dc.contributor.authorRettedal E
dc.contributor.authorKnowles SO
dc.contributor.authorRoy NC
dc.contributor.authorSjödin A
dc.contributor.authorWagner K-H
dc.contributor.authorO'Sullivan JM
dc.contributor.authorCameron-Smith D
dc.coverage.spatialEngland
dc.date.accessioned2024-10-30T21:28:48Z
dc.date.available2024-10-30T21:28:48Z
dc.date.issued2020-07-02
dc.description.abstractDiet has a major influence on the composition and metabolic output of the gut microbiome. Higher-protein diets are often recommended for older consumers; however, the effect of high-protein diets on the gut microbiota and faecal volatile organic compounds (VOC) of elderly participants is unknown. The purpose of the study was to establish if the faecal microbiota composition and VOC in older men are different after a diet containing the recommended dietary intake (RDA) of protein compared with a diet containing twice the RDA (2RDA). Healthy males (74⋅2 (sd 3⋅6) years; n 28) were randomised to consume the RDA of protein (0⋅8 g protein/kg body weight per d) or 2RDA, for 10 weeks. Dietary protein was provided via whole foods rather than supplementation or fortification. The diets were matched for dietary fibre from fruit and vegetables. Faecal samples were collected pre- and post-intervention for microbiota profiling by 16S ribosomal RNA amplicon sequencing and VOC analysis by head space/solid-phase microextraction/GC-MS. After correcting for multiple comparisons, no significant differences in the abundance of faecal microbiota or VOC associated with protein fermentation were evident between the RDA and 2RDA diets. Therefore, in the present study, a twofold difference in dietary protein intake did not alter gut microbiota or VOC indicative of altered protein fermentation.
dc.description.confidentialfalse
dc.format.paginatione25-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/32742642
dc.identifier.citationMitchell SM, McKenzie EJ, Mitchell CJ, Milan AM, Zeng N, D'Souza RF, Ramzan F, Sharma P, Rettedal E, Knowles SO, Roy NC, Sjödin A, Wagner K-H, O'Sullivan JM, Cameron-Smith D. (2020). A period of 10 weeks of increased protein consumption does not alter faecal microbiota or volatile metabolites in healthy older men: a randomised controlled trial.. J Nutr Sci. 9. (pp. e25-).
dc.identifier.doi10.1017/jns.2020.15
dc.identifier.eissn2048-6790
dc.identifier.elements-typejournal-article
dc.identifier.issn2048-6790
dc.identifier.numbere25
dc.identifier.piiS2048679020000154
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71875
dc.languageeng
dc.publisherCambridge University Press on behalf of The Nutrition Society
dc.publisher.urihttps://www.cambridge.org/core/journals/journal-of-nutritional-science/article/period-of-10-weeks-of-increased-protein-consumption-does-not-alter-faecal-microbiota-or-volatile-metabolites-in-healthy-older-men-a-randomised-controlled-trial/B86697AB9E27BCFD9F1DAB30943868B9#article
dc.relation.isPartOfJ Nutr Sci
dc.rights(c) 2020 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject16S amplicon sequencing
dc.subject16S rRNA, 16S ribosomal RNA
dc.subject2RDA, diet containing twice the RDA of protein
dc.subjectAMDIS, Automated Mass Spectral Deconvolution and Identification System
dc.subjectBCFA, branched-chain fatty acid
dc.subjectFermentation
dc.subjectHS/SPME/GCMS, head space/solid-phase microextraction/GC-MS
dc.subjectMaAsLin, Multivariate Association with Linear Model
dc.subjectMicrobiome
dc.subjectRDA, diet containing the RDA of protein
dc.subjectRecommended dietary intake
dc.subjectVOC, volatile organic compound
dc.subjectVolatile organic compounds
dc.subjectAged
dc.subjectDiet, High-Protein
dc.subjectDietary Proteins
dc.subjectFeces
dc.subjectGastrointestinal Microbiome
dc.subjectHumans
dc.subjectMale
dc.subjectMicrobiota
dc.subjectNutritional Requirements
dc.subjectTreatment Outcome
dc.subjectVolatile Organic Compounds
dc.titleA period of 10 weeks of increased protein consumption does not alter faecal microbiota or volatile metabolites in healthy older men: a randomised controlled trial
dc.typeJournal article
pubs.elements-id434461
pubs.organisational-groupOther
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