Optical Detection of CoV-SARS-2 Viral Proteins to Sub-Picomolar Concentrations

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dc.citation.issue9
dc.citation.volume6
dc.contributor.authorStanborough T
dc.contributor.authorGiven FM
dc.contributor.authorKoch B
dc.contributor.authorSheen CR
dc.contributor.authorStowers-Hull AB
dc.contributor.authorWaterland MR
dc.contributor.authorCrittenden DL
dc.coverage.spatialUnited States
dc.date.accessioned2024-01-25T23:52:14Z
dc.date.accessioned2024-07-25T06:42:18Z
dc.date.available2021-02-23
dc.date.available2024-01-25T23:52:14Z
dc.date.available2024-07-25T06:42:18Z
dc.date.issued2021-03-09
dc.description.abstractThe emergence of a new strain of coronavirus in late 2019, SARS-CoV-2, led to a global pandemic in 2020. This may have been preventable if large scale, rapid diagnosis of active cases had been possible, and this has highlighted the need for more effective and efficient ways of detecting and managing viral infections. In this work, we investigate three different optical techniques for quantifying the binding of recombinant SARS-CoV-2 spike protein to surface-immobilized oligonucleotide aptamers. Biolayer interferometry is a relatively cheap, robust, and rapid method that only requires very small sample volumes. However, its detection limit of 250 nM means that it is not sensitive enough to detect antigen proteins at physiologically relevant levels (sub-pM). Surface plasmon resonance is a more sensitive technique but requires larger sample volumes, takes longer, requires more expensive instrumentation, and only reduces the detection limit to 5 nM. Surface-enhanced Raman spectroscopy is far more sensitive, enabling detection of spike protein to sub-picomolar concentrations. Control experiments performed using scrambled aptamers and using bovine serum albumin as an analyte show that this apta-sensing approach is both sensitive and selective, with no appreciable response observed for any controls. Overall, these proof-of-principle results demonstrate that SERS-based aptasensors hold great promise for development into rapid, point-of-use antigen detection systems, enabling mass testing without any need for reagents or laboratory expertise and equipment.
dc.description.confidentialfalse
dc.format.pagination6404-6413
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/33718731
dc.identifier.citationStanborough T, Given FM, Koch B, Sheen CR, Stowers-Hull AB, Waterland MR, Crittenden DL. (2021). Optical Detection of CoV-SARS-2 Viral Proteins to Sub-Picomolar Concentrations.. ACS Omega. 6. 9. (pp. 6404-6413).
dc.identifier.doi10.1021/acsomega.1c00008
dc.identifier.eissn2470-1343
dc.identifier.elements-typejournal-article
dc.identifier.issn2470-1343
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/70719
dc.languageeng
dc.publisherAmerican Chemical Society
dc.publisher.urihttps://pubs.acs.org/doi/10.1021/acsomega.1c00008
dc.relation.isPartOfACS Omega
dc.rights(c) 2021 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleOptical Detection of CoV-SARS-2 Viral Proteins to Sub-Picomolar Concentrations
dc.typeJournal article
pubs.elements-id441856
pubs.organisational-groupOther
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