New insight into avian malaria vectors in New Zealand

Simple item page
dc.citation.issue1
dc.citation.volume17
dc.contributor.authorSchoener ER
dc.contributor.authorTompkins DM
dc.contributor.authorHowe L
dc.contributor.authorCastro IC
dc.coverage.spatialEngland
dc.date.accessioned2024-04-09T20:34:51Z
dc.date.accessioned2024-07-25T06:52:10Z
dc.date.available2024-03-22
dc.date.available2024-04-09T20:34:51Z
dc.date.available2024-07-25T06:52:10Z
dc.date.issued2024-03-22
dc.description.abstractBACKGROUND: Mosquitoes (Culicidae) are vectors for most malaria parasites of the Plasmodium species and are required for Plasmodium spp. to complete their life cycle. Despite having 16 species of mosquitoes and the detection of many Plasmodium species in birds, little is known about the role of different mosquito species in the avian malaria life cycle in New Zealand. METHODS: In this study, we used nested polymerase chain reaction (PCR) and real-time PCR to determine Plasmodium spp. prevalence and diversity of mitochondrial cytochrome b gene sequences in wild-caught mosquitoes sampled across ten sites on the North Island of New Zealand during 2012-2014. The mosquitoes were pooled by species and location collected, and the thorax and abdomens were examined separately for Plasmodium spp. DNA. Akaike information criterion (AIC) modeling was used to test whether location, year of sampling, and mosquito species were significant predictors of minimum infection rates (MIR). RESULTS: We collected 788 unengorged mosquitoes of six species, both native and introduced. The most frequently caught mosquito species were the introduced Aedes notoscriptus and the native Culex pervigilans. Plasmodium sp DNA was detected in 37% of matched thorax and abdomen pools. When considered separately, 33% of abdomen and 23% of thorax pools tested positive by nested PCR. The MIR of the positive thorax pools from introduced mosquito species was 1.79% for Ae. notoscriptus and 0% for Cx. quinquefasciatus, while the MIR for the positive thorax pools of native mosquito species was 4.9% for Cx. pervigilans and 0% for Opifex fuscus. For the overall MIR, site and mosquito species were significant predictors of Plasmodium overall MIR. Aedes notoscriptus and Cx. pervigilans were positive for malaria DNA in the thorax samples, indicating that they may play a role as avian malaria vectors. Four different Plasmodium lineages (SYAT05, LINN1, GRW6, and a new lineage of P (Haemamoeba) sp. AENOT11) were identified in the pooled samples. CONCLUSIONS: This is the first detection of avian Plasmodium DNA extracted from thoraxes of native Culex and introduced Aedes mosquito species in New Zealand and therefore the first study providing an indication of potential vectors in this country.
dc.description.confidentialfalse
dc.edition.editionDecember 2024
dc.format.pagination150-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/38519966
dc.identifier.citationSchoener ER, Tompkins DM, Howe L, Castro IC. (2024). New insight into avian malaria vectors in New Zealand.. Parasit Vectors. 17. 1. (pp. 150-).
dc.identifier.doi10.1186/s13071-024-06196-7
dc.identifier.eissn1756-3305
dc.identifier.elements-typejournal-article
dc.identifier.issn1756-3305
dc.identifier.number150
dc.identifier.pii10.1186/s13071-024-06196-7
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71068
dc.languageeng
dc.publisherBioMed Central Ltd
dc.publisher.urihttps://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-024-06196-7
dc.relation.isPartOfParasit Vectors
dc.rights(c) The author/sen
dc.rights.licenseCC BY 4.0en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectAedes
dc.subjectCulex
dc.subjectPlasmodium
dc.subjectMosquito
dc.subjectAnimals
dc.subjectMalaria, Avian
dc.subjectAnopheles
dc.subjectNew Zealand
dc.subjectMalaria
dc.subjectMosquito Vectors
dc.subjectCulex
dc.subjectPlasmodium
dc.subjectAedes
dc.subjectBirds
dc.subjectDNA, Protozoan
dc.titleNew insight into avian malaria vectors in New Zealand
dc.typeJournal article
pubs.elements-id488026
pubs.organisational-groupOther
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Published
Size:
1.79 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Journal Articles