Genetic regulation of antibody responsiveness to immunization in substrains of BALB/c mice.

Simple item page
dc.citation.issue1
dc.citation.volume97
dc.contributor.authorPoyntz HC
dc.contributor.authorJones A
dc.contributor.authorJauregui R
dc.contributor.authorYoung W
dc.contributor.authorGestin A
dc.contributor.authorMooney A
dc.contributor.authorLamiable O
dc.contributor.authorAltermann E
dc.contributor.authorSchmidt A
dc.contributor.authorGasser O
dc.contributor.authorWeyrich L
dc.contributor.authorJolly CJ
dc.contributor.authorLinterman MA
dc.contributor.authorGros GL
dc.contributor.authorHawkins ED
dc.contributor.authorForbes-Blom E
dc.coverage.spatialUnited States
dc.date.accessioned2025-04-15T23:06:27Z
dc.date.available2025-04-15T23:06:27Z
dc.date.issued2019-01-01
dc.description.abstractAntibody-mediated immunity is highly protective against disease. The majority of current vaccines confer protection through humoral immunity, but there is high variability in responsiveness across populations. Identifying immune mechanisms that mediate low antibody responsiveness may provide potential strategies to boost vaccine efficacy. Here, we report diverse antibody responsiveness to unadjuvanted as well as adjuvanted immunization in substrains of BALB/c mice, resulting in high and low antibody response phenotypes. Furthermore, these antibody phenotypes were not affected by changes in environmental factors such as the gut microbiota composition. Antigen-specific B cells following immunization had a marked difference in capability to class switch, resulting in perturbed IgG isotype antibody production. In vitro, a B-cell intrinsic defect in the regulation of class-switch recombination was identified in mice with low IgG antibody production. Whole genome sequencing identified polymorphisms associated with the magnitude of antibody produced, and we propose candidate genes that may regulate isotype class-switching capability. This study highlights that mice sourced from different vendors can have significantly altered humoral immune response profiles, and provides a resource to interrogate genetic regulators of antibody responsiveness. Together these results further our understanding of immune heterogeneity and suggest additional research on the genetic influences of adjuvanted vaccine strategies is warranted for enhancing vaccine efficacy.
dc.description.confidentialfalse
dc.edition.editionJanuary 2019
dc.format.pagination39-53
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/30152893
dc.identifier.citationPoyntz HC, Jones A, Jauregui R, Young W, Gestin A, Mooney A, Lamiable O, Altermann E, Schmidt A, Gasser O, Weyrich L, Jolly CJ, Linterman MA, Gros GL, Hawkins ED, Forbes-Blom E. (2019). Genetic regulation of antibody responsiveness to immunization in substrains of BALB/c mice.. Immunol Cell Biol. 97. 1. (pp. 39-53).
dc.identifier.doi10.1111/imcb.12199
dc.identifier.eissn1440-1711
dc.identifier.elements-typejournal-article
dc.identifier.issn0818-9641
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/72801
dc.languageeng
dc.publisherJohn Wiley and Sons Inc.
dc.publisher.urihttps://onlinelibrary.wiley.com/doi/10.1111/imcb.12199
dc.relation.isPartOfImmunol Cell Biol
dc.rights(c) 2018 Malaghan Institute of Medical Research
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectClass-switch recombination
dc.subjectgene regulation in immune cells
dc.subjecthumoral immunity
dc.subjectvaccines
dc.subjectAnimals
dc.subjectAntibody Formation
dc.subjectB-Lymphocytes
dc.subjectImmunoglobulin Class Switching
dc.subjectMice
dc.subjectMice, Inbred BALB C
dc.subjectPolymorphism, Genetic
dc.subjectVaccines
dc.subjectWhole Genome Sequencing
dc.titleGenetic regulation of antibody responsiveness to immunization in substrains of BALB/c mice.
dc.typeJournal article
pubs.elements-id486146
pubs.organisational-groupOther
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