An investigation into the use of cobalt chelates in peptide synthesis : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science, Massey University, Palmerston North.

Simple item page
dc.contributor.authorFriar, Murray John
dc.date.accessioned2018-03-09T01:35:02Z
dc.date.available2018-03-09T01:35:02Z
dc.date.issued1979
dc.description.abstractPreparative scale samples of [ Co(en)2 C12 ]C1 using the method of Bailar (27) and [ Co(en)2 Co3 ]Cl from CoCl6 were produced. The carbonate complex was also prepared from [ Co(en)2 C12 ]C1, using the method described by Buckingham (21). [ Co(en)2 C03 ]Cl was then converted to [ Co(en)2 Br2 ]Br. Alanato, Phenylalanato, NĪµnitroarginato, Valinato, Prolinato and O-Benzylaspatato complexes were synthesised from [Co(en)2 Br2 ]Br using a modification of Meisenheimers method outlined by Dekkers (26). Isoleucinato, Phenylalaninato,NĪµ nitroarginato, O-benzyl-aspatato and O-benzyltyrosinato complexes were also prepared under non solution conditions described by Dekkers (26). The formation of glycinatobis(ethylene-diamine)cobalt(III) complex preparation by a modified Meisenheimer method, was used as model reactions to optimise the source of base, pH conditions, solvent conditions and reaction times. The use of methyltri- flouromethane sulfonic acid and triflouromethane sulfonic acid in methanol, to alkylate the amino-acido-bis(ethylene-diamine)cobalt(III) complex was demonstrated. The conditions required to optimise the yield of the condensation of these methylated amino acid cobalt(III) with amino acid esters or peptide esters, was established, and the conditions necessary for the rapid ion exchange separation of the products investigated. The most suitable of the methods for the removal of the peptide from the cobalt(III) complexes, described by Dekkers, was established and Gel Filtration separation of the peptide products demonstrated. Problems with the instability of some peptide complexes, especially Co(en)2 PhePheOC6 H5 , were encountered and possible solutions tested. The synthesis using cobalt chelates of the amino acids of PhePheOC6 H5 , ProPheOC6 H5 , AlaPheOC6 H5 , ProPhePheOC6 H5 and AlaGlyGlyOC2 H5 had thus been attempted and the isolated products were submitted for amino.en_US
dc.identifier.urihttp://hdl.handle.net/10179/12947
dc.language.isoenen_US
dc.publisherMassey Universityen_US
dc.rightsThe Authoren_US
dc.subjectPeptide synthesisen_US
dc.subjectOrganocobalt compoundsen_US
dc.titleAn investigation into the use of cobalt chelates in peptide synthesis : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science, Massey University, Palmerston North.en_US
dc.typeThesisen_US
massey.contributor.authorFriar, Murray John
thesis.degree.disciplineChemistryen_US
thesis.degree.grantorMassey Universityen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Science (M. Sc.)en_US
Files
Original bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
01_front.pdf
Size:
4.06 MB
Format:
Adobe Portable Document Format
Description:
Download
Thumbnail Image
Name:
02_whole.pdf
Size:
21.44 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
3.32 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Theses and Dissertations