Ileal Digestibility of Nitrogen and Amino Acids in Human Milk and an Infant Formula as Determined in Neonatal Minipiglets

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dc.citation.issue4
dc.citation.volume153
dc.contributor.authorCharton E
dc.contributor.authorHenry G
dc.contributor.authorCahu A
dc.contributor.authorLe Gouar Y
dc.contributor.authorDahirel P
dc.contributor.authorMoughan PJ
dc.contributor.authorMontoya CA
dc.contributor.authorBellanger A
dc.contributor.authorDupont D
dc.contributor.authorLe Huërou-Luron I
dc.contributor.authorDeglaire A
dc.coverage.spatialUnited States
dc.date.accessioned2024-10-17T23:58:40Z
dc.date.available2024-10-17T23:58:40Z
dc.date.issued2023-04
dc.description.abstractBACKGROUND: Infant formula (IF) has to provide at least the same amount of amino acids (AAs) as human milk (HM). AA digestibility in HM and IF was not studied extensively, with no data available for tryptophan digestibility. OBJECTIVES: The present study aimed to measure the true ileal digestibility (TID) of total nitrogen and AAs in HM and IF to estimate AA bioavailability using Yucatan mini-piglets as an infant model. METHODS: Twenty-four 19-day-old piglets (males and females) received either HM or IF for 6 days or a protein-free diet for 3 days, with cobalt-EDTA as an indigestible marker. Diets were fed hourly over 6 h before euthanasia and digesta collection. Total N, AA, and marker contents in diets and digesta were measured to determine the TID. Unidimensional statistical analyses were conducted. RESULTS: Dietary N content was not different between HM and IF, while true protein was lower in HM (-4 g/L) due to a 7-fold higher non-protein N content in HM. The TID of total N was lower (P < 0.001) for HM (91.3 ± 1.24%) than for IF (98.0 ± 0.810%), while the TID of amino acid nitrogen (AAN) was not different (average of 97.4 ± 0.655%, P = 0.272). HM and IF had similar (P > 0.05) TID for most of the AAs including tryptophan (96.7 ± 0.950%, P = 0.079), except for some AAs (lysine, phenylalanine, threonine, valine, alanine, proline, and serine), with small significant difference (P < 0.05). The first limiting AA was the aromatic AAs, and the digestible indispensable AA score (DIAAS) was higher for HM (DIAASHM = 101) than for IF (DIAASIF = 83). CONCLUSION: HM, compared to IF, had a lower TID for total N only, whereas the TID of AAN and most AAs, including Trp, was high and similar. A larger proportion of non-protein N is transferred to the microbiota with HM, which is of physiological relevance, although this fraction is poorly considered for IF manufacturing.
dc.description.confidentialfalse
dc.edition.editionApril 2023
dc.format.pagination1063-1074
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/36868512
dc.identifier.citationCharton E, Henry G, Cahu A, Le Gouar Y, Dahirel P, Moughan PJ, Montoya CA, Bellanger A, Dupont D, Le Huërou-Luron I, Deglaire A. (2023). Ileal Digestibility of Nitrogen and Amino Acids in Human Milk and an Infant Formula as Determined in Neonatal Minipiglets.. J Nutr. 153. 4. (pp. 1063-1074).
dc.identifier.doi10.1016/j.tjnut.2023.02.025
dc.identifier.eissn1541-6100
dc.identifier.elements-typejournal-article
dc.identifier.issn0022-3166
dc.identifier.piiS0022-3166(23)12716-7
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71755
dc.languageeng
dc.publisherElsevier Inc. on behalf of American Society for Nutrition
dc.publisher.urihttps://www.sciencedirect.com/science/article/pii/S0022316623127167
dc.relation.isPartOfJ Nutr
dc.rights(c) 2023 The Author/s
dc.rightsCC BY-NC-ND 4.0
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectbioavailability
dc.subjectdigestible indispensable AA score
dc.subjecthuman milk
dc.subjectinfant nutrition
dc.subjectmultiple hydrolysis
dc.subjecttrue digestibility
dc.subjecttryptophan
dc.subjectMale
dc.subjectInfant, Newborn
dc.subjectInfant
dc.subjectFemale
dc.subjectHumans
dc.subjectAnimals
dc.subjectSwine
dc.subjectAmino Acids
dc.subjectMilk, Human
dc.subjectInfant Formula
dc.subjectTryptophan
dc.subjectNitrogen
dc.subjectDigestion
dc.subjectIleum
dc.subjectDiet
dc.subjectDiet, Protein-Restricted
dc.subjectAnimal Feed
dc.titleIleal Digestibility of Nitrogen and Amino Acids in Human Milk and an Infant Formula as Determined in Neonatal Minipiglets
dc.typeJournal article
pubs.elements-id460689
pubs.organisational-groupOther
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