In vitro synergy of 5-nitrofurans, vancomycin and sodium deoxycholate against Gram-negative pathogens

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dc.citation.issue3
dc.citation.volume70
dc.contributor.authorOlivera C
dc.contributor.authorLe VVH
dc.contributor.authorDavenport C
dc.contributor.authorRakonjac J
dc.coverage.spatialEngland
dc.date.accessioned2024-01-30T01:04:25Z
dc.date.accessioned2024-07-25T06:47:19Z
dc.date.available2021-01-15
dc.date.available2024-01-30T01:04:25Z
dc.date.available2024-07-25T06:47:19Z
dc.date.issued2021-01-15
dc.description.abstractIntroduction. There is an urgent need for effective therapies against bacterial infections, especially those caused by antibiotic-resistant Gram-negative pathogens. Hypothesis. Synergistic combinations of existing antimicrobials show promise due to their enhanced efficacies and reduced dosages which can mitigate adverse effects, and therefore can be used as potential antibacterial therapy. Aim. In this study, we sought to characterize the in vitro interaction of 5-nitrofurans, vancomycin and sodium deoxycholate (NVD) against pathogenic bacteria. Methodology. The synergy of the NVD combination was investigated in terms of growth inhibition and bacterial killing using checkerboard and time-kill assays, respectively. Results. Using a three-dimensional checkerboard assay, we showed that 5-nitrofurans, sodium deoxycholate and vancomycin interact synergistically in the growth inhibition of 15 out of 20 Gram-negative strains tested, including clinically significant pathogens such as carbapenemase-producing Escherichia coli, Klebsiella pneumoniae and Acinetobacter baumannii, and interact indifferently against the Gram-positive strains tested. The time-kill assay further confirmed that the triple combination was bactericidal in a synergistic manner. Conclusion. This study demonstrates the synergistic effect of 5-nitrofurans, sodium deoxycholate and vancomycin against Gram-negative pathogens and highlights the potential of the combination as a treatment for Gram-negative and Gram-positive infections.
dc.description.confidentialfalse
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/33448923
dc.identifier.citationOlivera C, Le VVH, Davenport C, Rakonjac J. (2021). In vitro synergy of 5-nitrofurans, vancomycin and sodium deoxycholate against Gram-negative pathogens.. J Med Microbiol. 70. 3.
dc.identifier.doi10.1099/jmm.0.001304
dc.identifier.eissn1473-5644
dc.identifier.elements-typejournal-article
dc.identifier.issn0022-2615
dc.identifier.numberARTN 001304
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/70876
dc.languageeng
dc.publisherMicrobiology Society
dc.publisher.urihttps://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.001304
dc.relation.isPartOfJ Med Microbiol
dc.rightsCC BY-NC 4.0
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/legalcode
dc.subjectantibiotic resistance
dc.subjectenterobacteria
dc.subjectnitrofurans
dc.subjectsodium deoxycholate
dc.subjectsynergy
dc.subjectvancomycin
dc.subjectAnti-Bacterial Agents
dc.subjectDeoxycholic Acid
dc.subjectDrug Resistance, Multiple, Bacterial
dc.subjectDrug Synergism
dc.subjectGram-Negative Bacteria
dc.subjectNitrofurans
dc.subjectVancomycin
dc.titleIn vitro synergy of 5-nitrofurans, vancomycin and sodium deoxycholate against Gram-negative pathogens
dc.typeJournal article
pubs.elements-id438472
pubs.organisational-groupOther
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