Forget-me-not phylogenomics: Improving the resolution and taxonomy of a rapid island and mountain radiation in Aotearoa New Zealand (Myosotis; Boraginaceae)

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dc.citation.volume204
dc.contributor.authorMeudt HM
dc.contributor.authorPearson S
dc.contributor.authorNing W
dc.contributor.authorPrebble JM
dc.contributor.authorTate JA
dc.coverage.spatialUnited States
dc.date.accessioned2024-12-09T21:05:39Z
dc.date.available2024-12-09T21:05:39Z
dc.date.issued2024-11-22
dc.description.abstractIsland and mountain systems represent natural laboratories for studies of species radiations, but they often present several challenges for phylogenetic inference and species delimitation. The southern hemisphere forget-me-nots (Myosotis, Boraginaceae) comprise a geologically recent radiation centred in New Zealand, a mountainous archipelago, with about 50 species that are morphologically and ecologically divergent but lack genetic variation sufficient to resolve phylogenetic relationships and species boundaries using standard DNA Sanger sequencing markers, AFLPs, or microsatellites. Many of these Myosotis species are geographically restricted in alpine areas, uncommon or threatened, have polyploid and dysploid genomes, and are of high taxonomic and conservation priority. Here we present phylogenomic analyses using target-capture of Angiosperms353 baits, and genome skimming of whole plastomes and nrDNA, to improve resolution of the radiation, explore biogeographic and morphological patterns within it, and address specific taxonomic questions for each species. Our comprehensive sampling includes over 300 individuals representing nearly all species from Aotearoa New Zealand and Australia, which is ∼ 2-3 × more taxon sampling and ∼ 80-120 × more molecular data than previously published for Myosotis. Exploration of different data filtering, curation and analyses (coalescent vs. concatenation) improved the resolution of the Angiosperms353 tree, which despite short backbone branches with low support values, showed taxonomic and geographic patterns, including multiple switches between ebracteate and bracteate inflorescences and multiple expansions within New Zealand from Te Waipounamu South Island to Te Ika-a-Māui North Island, Rakiura Stewart Island, subantarctic islands, and Australia. Some of these patterns were also seen in the genome skimming datasets, and comparison of the three datasets was useful for improving our understanding of the taxonomy and resolution of this radiation. Although this phylogenomic study does not fully overcome all of the challenges regarding species delimitation of this rapid island and mountain species radiation, it nevertheless makes an important contribution to an integrative taxonomic revision of the southern hemisphere species of Myosotis.
dc.description.confidentialfalse
dc.edition.edition2025
dc.format.pagination108250-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/39581357
dc.identifier.citationMeudt HM, Pearson S, Ning W, Prebble JM, Tate JA. (2024). Forget-me-not phylogenomics: Improving the resolution and taxonomy of a rapid island and mountain radiation in Aotearoa New Zealand (Myosotis; Boraginaceae).. Mol Phylogenet Evol. 204. (pp. 108250-).
dc.identifier.doi10.1016/j.ympev.2024.108250
dc.identifier.eissn1095-9513
dc.identifier.elements-typejournal-article
dc.identifier.issn1055-7903
dc.identifier.number108250
dc.identifier.piiS1055-7903(24)00242-2
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/72243
dc.languageeng
dc.publisherElsevier Inc.
dc.publisher.urihttps://www.sciencedirect.com/science/article/pii/S1055790324002422
dc.relation.isPartOfMol Phylogenet Evol
dc.rights(c) The author/sen
dc.rights.licenseCC BYen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectAngiosperms353
dc.subjectBiogeography
dc.subjectGenome skimming
dc.subjectPlastome
dc.subjectSouthern hemisphere
dc.subjectSpecies delimitation
dc.titleForget-me-not phylogenomics: Improving the resolution and taxonomy of a rapid island and mountain radiation in Aotearoa New Zealand (Myosotis; Boraginaceae)
dc.typeJournal article
pubs.elements-id492441
pubs.organisational-groupOther
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