Immobilization of Horseradish Peroxidase and Myoglobin Using Sodium Alginate for Treating Organic Pollutants

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dc.citation.issue6
dc.citation.volume16
dc.contributor.authorWang X
dc.contributor.authorGhanizadeh H
dc.contributor.authorKhan S
dc.contributor.authorWu X
dc.contributor.authorLi H
dc.contributor.authorSadiq S
dc.contributor.authorLiu J
dc.contributor.authorLiu H
dc.contributor.authorYue Q
dc.contributor.editorHung Y-T
dc.date.accessioned2025-01-12T22:07:39Z
dc.date.available2025-01-12T22:07:39Z
dc.date.issued2024-03-15
dc.description.abstractRemoving organic pollutants from wastewater is crucial to prevent environmental contamination and protect human health. Immobilized enzymes are increasingly being explored for wastewater treatment due to their specific catalytic activities, reusability, and stability under various environmental conditions. Peroxidases, such as horseradish peroxidase (HRP) and myoglobin (Mb), are promising candidates for immobilized enzymes utilized in wastewater treatment due to their ability to facilitate the oxidation process of a wide range of organic molecules. However, the properties of the carrier and support materials greatly influence the stability and activity of immobilized HRP and Mb. In this research, we developed immobilized HRP and Mb using support material composed of sodium alginate and CaCl2 as carriers and glutaraldehyde as a crosslinking agent. Following this, the efficacy of immobilized HRP and Mb in removing aniline, phenol, and p-nitrophenol was assessed. Both immobilized enzymes removed all three organic pollutants from an aqueous solution, but Mb was more effective than HRP. After being immobilized, both enzymes became more resilient to changes in temperature and pH. Both immobilized enzymes retained their ability to eliminate organic pollutants through eight treatment cycles. Our study uncovered novel immobilized enzyme microspheres and demonstrated their successful application in wastewater treatment, paving the way for future research.
dc.description.confidentialfalse
dc.edition.editionMarch-2 2024
dc.identifier.citationWang X, Ghanizadeh H, Khan S, Wu X, Li H, Sadiq S, Liu J, Liu H, Yue Q. (2024). Immobilization of Horseradish Peroxidase and Myoglobin Using Sodium Alginate for Treating Organic Pollutants. Water (Switzerland). 16. 6.
dc.identifier.doi10.3390/w16060848
dc.identifier.eissn2073-4441
dc.identifier.elements-typejournal-article
dc.identifier.number848
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/72353
dc.languageEnglish
dc.publisherMDPI (Basel, Switzerland)
dc.publisher.urihttps://www.mdpi.com/2073-4441/16/6/848
dc.relation.isPartOfWater (Switzerland)
dc.rights(c) 2024 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectimmobilization
dc.subjectperoxidase
dc.subjectsodium alginate
dc.subjectreusability
dc.subjectorganic pollutants
dc.titleImmobilization of Horseradish Peroxidase and Myoglobin Using Sodium Alginate for Treating Organic Pollutants
dc.typeJournal article
pubs.elements-id488081
pubs.organisational-groupOther
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