Development of a qPCR molecular diagnostic assay for the detection of kiwi Eimeria species and its application to determine tissue-specificity of species causing coccidiosis in North Island brown kiwi (Apteryx mantelli)

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dc.citation.issue7
dc.citation.volume124
dc.contributor.authorScheltema E
dc.contributor.authorMorgan K
dc.contributor.authorHunter S
dc.contributor.authorMackay J
dc.contributor.authorSingh P
dc.contributor.authorHowe L
dc.date.accessioned2025-08-04T21:32:47Z
dc.date.available2025-08-04T21:32:47Z
dc.date.issued2025-07-04
dc.description.abstractJuvenile kiwi (Apteryx spp.) within captive-rearing programmes commonly suffer from coccidiosis, which primarily affects the intestine but can also impact other organs, such as the kidneys, liver, lung, and spleen (Morgan et al. Avian Pathol 42:137–146 2013). In some immune-compromised birds, disease causes significant morbidity and, occasionally, mortality (Morgan et al. NZVJ 62:315–320 2014); however, understanding of the biology of disease-causing Eimeria species in kiwi is limited. A probe-based qPCR assay targeting a 115-bp fragment of the Eimeria mitochondrial cytochrome c oxidase I (CO1) gene was developed to identify three distinct kiwi Eimeria species: the two species most commonly recovered from faeces, Eimeria kiwii and Eimeria apteryxii, as well as the newly described species, Eimeria koka (Scheltema et al. Syst Parasitol 102:30 2025). The qPCR assay was then applied to retrospectively analyse formalin-fixed paraffin-embedded intestine, kidney, liver, lung, and spleen tissues from ten historic post-mortem cases from kiwi diagnosed with extraintestinal coccidiosis. This novel assay detected infection more often (33/47 tissues) than manual histopathological identification (25/47 tissues). Only one species, E. koka, was detected in extraintestinal tissues with the highest prevalence (9/10) in kidney tissues. In contrast, E. kiwii was reliably detected in 8/9 intestinal tissues but was not detected in the other tissues tested. E. apteryxii was not detected in any of the tissues analysed. These findings suggest that kiwi are infected by at least one intestinal and one renal-specific species, the latter of which is suspected to disseminate under certain conditions to other organs of the body.
dc.description.confidentialfalse
dc.edition.editionJuly 2025
dc.identifier.citationScheltema E, Morgan K, Hunter S, Mackay J, Singh P, Howe L. (2025). Development of a qPCR molecular diagnostic assay for the detection of kiwi Eimeria species and its application to determine tissue-specificity of species causing coccidiosis in North Island brown kiwi (Apteryx mantelli). Parasitology Research. 124. 7.
dc.identifier.doi10.1007/s00436-025-08521-0
dc.identifier.eissn1432-1955
dc.identifier.elements-typejournal-article
dc.identifier.issn0932-0113
dc.identifier.number77
dc.identifier.piis00436-025-08521-0
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/73275
dc.languageEnglish
dc.publisherSpringer-Verlag GmbH Germany
dc.publisher.urihttps://link.springer.com/article/10.1007/s00436-025-08521-0
dc.relation.isPartOfParasitology Research
dc.rights(c) The author/sen
dc.rights.licenseOpen Access funding enabled and organized by CAUL and its Member Institutionsen
dc.rights.licenseCC BYen
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/en
dc.subjectEimeria
dc.subjectExtraintestinal infection
dc.subjectqPCR
dc.subjectCoccidiosis
dc.subjectCO1
dc.titleDevelopment of a qPCR molecular diagnostic assay for the detection of kiwi Eimeria species and its application to determine tissue-specificity of species causing coccidiosis in North Island brown kiwi (Apteryx mantelli)
dc.typeJournal article
pubs.elements-id501631
pubs.organisational-groupOther
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