Emetic toxin production of Bacillus cereus in a biofilm

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dc.citation.volume154
dc.contributor.authorHuang Y
dc.contributor.authorFlint SH
dc.contributor.authorLoo TS
dc.contributor.authorPalmer JS
dc.date.accessioned2023-10-19T22:57:52Z
dc.date.accessioned2023-10-25T20:49:32Z
dc.date.available2021-11-19
dc.date.available2023-10-19T22:57:52Z
dc.date.available2023-10-25T20:49:32Z
dc.date.issued2022-01-15
dc.date.updated2023-10-17T00:51:12Z
dc.description.abstractBacillus cereus sensu stricto (B. cereus) belongs to the B. cereus group, and is a well-known foodborne pathogen causing human disease including emesis which is caused by an emetic toxin, cereulide, with 105-108 cells per gram required to cause disease. The presence of this highly heat, pH and protease-resistant toxin presents a serious challenge to the food industry, as the bacteria itself may be eliminated during processing but the cereulide toxin will survive most food processing techniques. This study shows that cereulide toxin is associated with cells and biofilm structures rather than suspended in the surrounding liquid phase or environment. This is the first report investigating the cereulide toxin production in the presence of biofilms of B. cereus, showing that the cereulide toxin produced is associated with biofilm complex and also attaches to the substrate such as glass and stainless-steel on which the biofilm grows. The RT-qPCR showed that the expression of cesA and cesB were comparable between planktonic cells and biofilms. This study contributes a better understanding of food safety issues in the industry caused by cereulide toxin produced by B. cereus, and provides valuable information for developing control methods for cereulide toxin in the food industry.
dc.description.confidentialfalse
dc.identifierARTN 112840
dc.identifierS0023643821019939
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000735318300005&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=c5bb3b2499afac691c2e3c1a83ef6fef
dc.identifier.citationHuang Y, Flint SH, Loo TS, Palmer JS. (2022). Emetic toxin production of Bacillus cereus in a biofilm. LWT. 154.
dc.identifier.doi10.1016/j.lwt.2021.112840
dc.identifier.eissn1096-1127
dc.identifier.elements-typejournal-article
dc.identifier.harvestedMassey_Dark
dc.identifier.issn0023-6438
dc.identifier.urihttp://hdl.handle.net/10179/20389
dc.languageEnglish
dc.publisherElsevier Ltd
dc.publisher.urihttps://www.sciencedirect.com/science/article/pii/S0023643821019939
dc.relation.isPartOfLWT
dc.rights(c) 2021 The Author/sen_US
dc.rightsCC BY-NC-NDen_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.subjectCereulide
dc.subjectLC-MS
dc.subjectMS
dc.subjectGlass wool
dc.subjectStainless-steel
dc.subjectAttachment
dc.titleEmetic toxin production of Bacillus cereus in a biofilm
dc.typeJournal article
pubs.elements-id449864
pubs.organisational-groupOther
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