20 years later: unravelling the genomic success of New Zealand’s home-grown AK3 community-associated methicillin-resistant Staphylococcus aureus

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dc.citation.issue7
dc.citation.volume11
dc.contributor.authorWhite RT
dc.contributor.authorBakker S
dc.contributor.authorBloomfield M
dc.contributor.authorBurton M
dc.contributor.authorElvy J
dc.contributor.authorEustace A
dc.contributor.authorFrench NP
dc.contributor.authorGrant J
dc.contributor.authorGreening SS
dc.contributor.authorGrinberg A
dc.contributor.authorHarland C
dc.contributor.authorHutton S
dc.contributor.authorKarkaba A
dc.contributor.authorMartin J
dc.contributor.authorMatthews B
dc.contributor.authorMiller H
dc.contributor.authorStraub C
dc.contributor.authorTarring C
dc.contributor.authorTaylor WT
dc.contributor.authorUssher J
dc.contributor.authorVelasco C
dc.contributor.authorVoss EM
dc.contributor.authorDyet K
dc.date.accessioned2025-08-18T03:00:21Z
dc.date.available2025-08-18T03:00:21Z
dc.date.issued2025-07-25
dc.description.abstractMethicillin-resistant Staphylococcus aureus (MRSA) represents a significant public health challenge. In New Zealand, the community-associated MRSA sequence type (ST)5, carrying the staphylococcal cassette chromosome mec (SCCmec) type IV genetic element (which confers methicillin resistance), has been predominant since its detection in 2005. Known informally as the AK3 strain, it also exhibits resistance to fusidic acid. Here, we investigated the genomic evolution of the AK3 strain by analysing 397 genomes, comprising 361 MRSA and 36 closely related methicillin-susceptible S. aureus (MSSA) genomes, including 285 recently sequenced isolates from New Zealand spanning 2020 (n=30), 2021 (n=77), 2022 (n=88), 2023 (n=73) and 2024 (n=17). Phylogenetic analysis revealed that the AK3 strain evolved through stepwise acquisition of mobile genetic elements, with an MSSA ancestor likely introduced to New Zealand in the late 1970s. The lineage first acquired a SaPITokyo12571-like pathogenicity island, which contains the staphylococcal enterotoxin C bovine variant (sec-bov) and an enterotoxin-like protein (sel), between 1984 and 1991. This was followed by the integration of SCCmec type IV and adjacent fusidic acid resistance operon between 1997 and 2000. This timing coincides with increased community fusidic acid use in New Zealand. The AK3 strain then diversified into three major clades, spreading throughout New Zealand and Australia, with sporadic detection in European countries and Samoa. Our findings demonstrate how the sequential acquisition of mobile genetic elements, combined with antibiotic selection pressure, likely contributed to the successful emergence of AK3 and its spread in the South Pacific region.
dc.description.confidentialfalse
dc.edition.edition2025
dc.identifier.citationWhite RT, Bakker S, Bloomfield M, Burton M, Elvy J, Eustace A, French NP, Grant J, Greening SS, Grinberg A, Harland C, Hutton S, Karkaba A, Martin J, Matthews B, Miller H, Straub C, Tarring C, Taylor W, Ussher J, Velasco C, Voss EM, Dyet K. (2025). 20 years later: unravelling the genomic success of New Zealand’s home-grown AK3 community-associated methicillin-resistant Staphylococcus aureus. Microbial Genomics. 11. 7.
dc.identifier.doi10.1099/mgen.0.001452
dc.identifier.eissn2057-5858
dc.identifier.elements-typejournal-article
dc.identifier.number001452
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/73371
dc.languageEnglish
dc.publisherMicrobiology Society
dc.publisher.urihttp://microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.001452
dc.relation.isPartOfMicrobial Genomics
dc.rights(c) 2025 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.en
dc.subjectantibiotic resistance
dc.subjectgenomic surveillance
dc.subjectinfection control
dc.subjectoutbreak detection
dc.subjectphylogenetic analysis
dc.title20 years later: unravelling the genomic success of New Zealand’s home-grown AK3 community-associated methicillin-resistant Staphylococcus aureus
dc.typeJournal article
pubs.elements-id502714
pubs.organisational-groupOther
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