Presence of Unabsorbed Free Amino Acids at the End of the Small Intestine Indicates the Potential for an Increase in Amino Acid Uptake in Humans and Pigs

Simple item page
dc.citation.issue3
dc.citation.volume153
dc.contributor.authorvan der Wielen N
dc.contributor.authorde Vries S
dc.contributor.authorGerrits WJ
dc.contributor.authorLammers-Jannink K
dc.contributor.authorMoughan PJ
dc.contributor.authorMensink M
dc.contributor.authorHendriks W
dc.coverage.spatialUnited States
dc.date.accessioned2024-10-14T22:39:32Z
dc.date.available2024-10-14T22:39:32Z
dc.date.issued2023-03
dc.description.abstractBACKGROUND: Unabsorbed free amino acids (AAs) at the end of the small intestine result in a potential preventable nutritional loss. OBJECTIVES: This study aimed to quantify free AAs in terminal ileal digesta of both humans and pigs to investigate its relevance for the nutritional value of food proteins. METHODS: Two studies with three diets were performed: a human study-ileal digesta from eight adult ileostomates were collected over 9 h after ingestion of a single meal unsupplemented or supplemented with 30 g zein or whey; pig study-12 cannulated pigs were fed for 7 d with a diet containing whey or zein or no-protein diet, and ileal digesta were collected on the last 2 d. Digesta were analyzed for total and 13 free AAs. True ileal digestibility (TID) of AAs was compared with and without free AAs. RESULTS: All terminal ileal digesta samples contained free AAs. The TID of AAs in whey was 97% ± 2.4% (mean ± SD) in human ileostomates and 97% ± 1.9% in growing pigs. If the analyzed free AAs would have been absorbed, TID of whey would increase by 0.4%-units in humans and 0.1%-units in pigs. The TID of AAs in zein was 70% ± 16.4% in humans and 77% ± 20.6% in pigs and would increase by 2.3%-units and 3.5%-units, respectively, if the analyzed free AAs would have been fully absorbed. The largest difference was observed for threonine from zein: if free threonine was absorbed, the TID would increase by 6.6%-units in both species (P < 0.05). CONCLUSIONS: Free AAs are present at the end of the small intestine and can potentially have a nutritionally relevant effect for poorly digestible protein sources, whereas the effect is negligible for highly digestible protein sources. This result provides insight into the room for improvement of a protein's nutritional value if all free AAs are to be absorbed. J Nutr 2023;xx:xx-xx. This trial was registered at clinicaltrials.gov as NCT04207372.
dc.description.confidentialfalse
dc.edition.editionMarch 2023
dc.format.pagination673-682
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/36809852
dc.identifier.citationvan der Wielen N, de Vries S, Gerrits WJ, Lammers-Jannink K, Moughan PJ, Mensink M, Hendriks W. (2023). Presence of Unabsorbed Free Amino Acids at the End of the Small Intestine Indicates the Potential for an Increase in Amino Acid Uptake in Humans and Pigs.. J Nutr. 153. 3. (pp. 673-682).
dc.identifier.doi10.1016/j.tjnut.2023.01.038
dc.identifier.eissn1541-6100
dc.identifier.elements-typejournal-article
dc.identifier.issn0022-3166
dc.identifier.piiS0022-3166(23)06592-6
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71712
dc.languageeng
dc.publisherElsevier Inc on behalf of American Society for Nutrition
dc.publisher.urihttps://www.sciencedirect.com/science/article/pii/S0022316623065926
dc.relation.isPartOfJ Nutr
dc.rights(c) The author/sen
dc.rights.licenseCC BYen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectamino acid absorption
dc.subjectamino acid bioavailability
dc.subjectfree amino acids
dc.subjectprotein quality
dc.subjecttrue ileal digestibility
dc.subjectAnimals
dc.subjectHumans
dc.subjectAmino Acids
dc.subjectAnimal Feed
dc.subjectAnimal Nutritional Physiological Phenomena
dc.subjectDiet
dc.subjectDigestion
dc.subjectIleum
dc.subjectSwine
dc.subjectThreonine
dc.subjectZein
dc.titlePresence of Unabsorbed Free Amino Acids at the End of the Small Intestine Indicates the Potential for an Increase in Amino Acid Uptake in Humans and Pigs
dc.typeJournal article
pubs.elements-id460690
pubs.organisational-groupOther
Files
Original bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
Published version.pdf
Size:
354.02 KB
Format:
Adobe Portable Document Format
Description:
460690 PDF.pdf
Download
Thumbnail Image
Name:
Evidence
Size:
29.26 KB
Format:
Microsoft Word XML
Description:
1-s2.0-S0022316623065926-mmc1.docx
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
9.22 KB
Format:
Plain Text
Description:
Download
Collections
Journal Articles