Chromosome conformation maps in fission yeast reveal cell cycle dependent sub nuclear structure

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dc.citation.issue20
dc.citation.volume42
dc.contributor.authorGrand RS
dc.contributor.authorPichugina T
dc.contributor.authorGehlen LR
dc.contributor.authorJones MB
dc.contributor.authorTsai P
dc.contributor.authorAllison JR
dc.contributor.authorMartienssen R
dc.contributor.authorO'Sullivan JM
dc.date.available2014-11-10
dc.date.issued2014-11-10
dc.descriptionC The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http: // creativecommons.org / licenses / by / 4.0 / ), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.description.abstractSuccessful progression through the cell cycle requires spatial and temporal regulation of gene transcript levels and the number, positions and condensation levels of chromosomes. Here we present a high resolution survey of genome interactions in Schizosaccharomyces pombe using synchronized cells to investigate cell cycle dependent changes in genome organization and transcription. Cell cycle dependent interactions were captured between and within S. pombe chromosomes. Known features of genome organization (e.g. the clustering of telomeres and retrotransposon long terminal repeats (LTRs)) were observed throughout the cell cycle. There were clear correlations between transcript levels and chromosomal interactions between genes, consistent with a role for interactions in transcriptional regulation at specific stages of the cell cycle. In silico reconstructions of the chromosome organization within the S. pombe nuclei were made by polymer modeling. These models suggest that groups of genes with high and low, or differentially regulated transcript levels have preferred positions within the S. pombe nucleus. We conclude that the S. pombe nucleus is spatially divided into functional sub-nuclear domains that correlate with gene activity. The observation that chromosomal interactions are maintained even when chromosomes are fully condensed in M phase implicates genome organization in epigenetic inheritance and bookmarking.
dc.description.publication-statusPublished
dc.format.extent12585 - 12599 (15)
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000347693200026&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=c5bb3b2499afac691c2e3c1a83ef6fef
dc.identifier.citationNUCLEIC ACIDS RESEARCH, 2014, 42 (20), pp. 12585 - 12599 (15)
dc.identifier.doi10.1093/nar/gku965
dc.identifier.elements-id218767
dc.identifier.harvestedMassey_Dark
dc.identifier.issn0305-1048
dc.languageEnglish
dc.publisherOxford University Press
dc.relation.isPartOfNUCLEIC ACIDS RESEARCH
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectBiochemistry & Molecular Biology
dc.subjectPOL III GENES
dc.subjectSCHIZOSACCHAROMYCES-POMBE
dc.subjectGENOME ORGANIZATION
dc.subjectTRANSCRIPTIONAL MEMORY
dc.subjectMETAPHASE CHROMOSOMES
dc.subjectREPLICATION
dc.subjectCHROMATIN
dc.subjectBOOKMARKING
dc.subjectRETROTRANSPOSONS
dc.subjectREORGANIZATION
dc.subject.anzsrc05 Environmental Sciences
dc.subject.anzsrc06 Biological Sciences
dc.subject.anzsrc08 Information and Computing Sciences
dc.titleChromosome conformation maps in fission yeast reveal cell cycle dependent sub nuclear structure
dc.typeJournal article
pubs.notesNot known
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Sciences
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