The development of polyester bead-based particulate subunit vaccine against Johne's disease : a thesis presented in partial fulfillment of the requirements of the degree of Master of Science in Microbiology, Massey University, Palmerston North, Institute of Fundamental Science

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dc.contributor.authorSitthirit, Panan
dc.date.accessioned2017-06-27T20:36:11Z
dc.date.available2017-06-27T20:36:11Z
dc.date.issued2015
dc.description.abstractJohne’s disease is the intestinal infection in ruminants caused by Mycobacterium avium subspecies paratuberculosis (MAP). The disease is economically important in the dairy industry as infection of the calves or mature cattle can result in death. Current vaccination as a part of disease control is not only partially protective against MAP but also interferes with current diagnostic test for bovine tuberculosis. Therefore, more effective and defined vaccines are needed. In this study, vaccine candidates were developed by bioengineering Escherichia coli to produce polyhydroxyalkanoate (PHA) beads displaying selected vaccine candidate antigens as fusion proteins. The selected antigens were the MAP 85 antigen complex (Ag85A and Ag85B), Superoxide dismutase (SOD) and a recombinant fusion protein 74F, however, only the antigenpresenting beads with truncated Ag85A, Ag85B and SOD were successfully produced and purified. The fusion protein comprising the respective antigens was identified and confirmed to be associated with PHA beads. The PHA beads were partially purified for future characterisations such as binding of antigen specific antibodies on PHA beads in vitro and immunological properties in animal modelsen_US
dc.identifier.urihttp://hdl.handle.net/10179/11363
dc.language.isoenen_US
dc.publisherMassey Universityen_US
dc.rightsThe Authoren_US
dc.subjectEscherichia colien_US
dc.subjectBiotechnologyen_US
dc.subjectParatuberculosisen_US
dc.subjectJohne's diseaseen_US
dc.subjectVeterinary vaccinesen_US
dc.subjectMycobacterium avium paratuberculosisen_US
dc.titleThe development of polyester bead-based particulate subunit vaccine against Johne's disease : a thesis presented in partial fulfillment of the requirements of the degree of Master of Science in Microbiology, Massey University, Palmerston North, Institute of Fundamental Scienceen_US
dc.typeThesisen_US
massey.contributor.authorSitthirit, Pananen_US
thesis.degree.disciplineMicrobiologyen_US
thesis.degree.grantorMassey Universityen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Science (MSc)en_US
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