Characterisation of malate-dependent mutants of the yeast Schizosaccharomyces malidevorans : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Genetics at Massey University

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dc.contributor.authorHansen, Nicolette Olivia
dc.date.accessioned2019-04-30T02:46:44Z
dc.date.available2019-04-30T02:46:44Z
dc.date.issued1991
dc.description.abstractThe phenotypes of several UV induced malate-dependent mutants of Schizosaccharomyces malidevorans were characterised and compared to the previously characterised malate-dependent strain, S. malidevorans #11. This strain uses less glucose than the wild type, has an extended life in liquid media, does not sporulate readily and turns indicator medium blue. The malate-dependent mutant strains were analysed for their fermentation characteristics, their complementation groups and their chromosomal patterns on a transverse alternating field electrophoresis apparatus (TAFE). The fermentation patterns of all of the strains were similar to #11. There appear to be three complementation groups involved in the malate-dependent phenotype. The TAFE patterns and subsequent Southern Hybridisation showed that the malateĀ­ dependent mutants had a decreased mobility of chromosome II, while chromosomes I and III were not altered. The chromosome II alteration varied between the different malate-dependent mutants but fell into a small number of discrete patterns.en_US
dc.identifier.urihttp://hdl.handle.net/10179/14545
dc.language.isoenen_US
dc.publisherMassey Universityen_US
dc.rightsThe Authoren_US
dc.subjectYeast fungien_US
dc.subjectGeneticsen_US
dc.subjectGenetic engineeringen_US
dc.subjectBiotechnologyen_US
dc.titleCharacterisation of malate-dependent mutants of the yeast Schizosaccharomyces malidevorans : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Genetics at Massey Universityen_US
dc.typeThesisen_US
massey.contributor.authorHansen, Nicolette Olivia
thesis.degree.disciplineGeneticsen_US
thesis.degree.grantorMassey Universityen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Science (M. Sc.)en_US
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