Slowed gastrointestinal transit is associated with an altered caecal microbiota in an aged rat model

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dc.citation.volume13
dc.contributor.authorParkar N
dc.contributor.authorDalziel JE
dc.contributor.authorSpencer NJ
dc.contributor.authorJanssen P
dc.contributor.authorMcNabb WC
dc.contributor.authorYoung W
dc.contributor.editorButcher J
dc.coverage.spatialSwitzerland
dc.date.accessioned2024-10-20T19:28:57Z
dc.date.available2024-10-20T19:28:57Z
dc.date.issued2023-03-14
dc.description.abstractGastrointestinal (GI) motility is largely dependent upon activity within the enteric nervous system (ENS) and is an important part of the digestive process. Dysfunction of the ENS can impair GI motility as is seen in the case of constipation where gut transit time is prolonged. Animal models mimicking symptoms of constipation have been developed by way of pharmacological manipulations. Studies have reported an association between altered GI motility and gut microbial population. Little is known about the changes in gut microbiota profile resulting specifically from pharmacologically induced slowed GI motility in rats. Moreover, the relationship between gut microbiota and altered intestinal motility is based on studies using faecal samples, which are easier to obtain but do not accurately reflect the intestinal microbiome. The aim of this study was to examine how delayed GI transit due to opioid receptor agonism in the ENS modifies caecal microbiota composition. Differences in caecal microbial composition of loperamide-treated or control male Sprague Dawley rats were determined by 16S rRNA gene amplicon sequencing. The results revealed that significant differences were observed at both genus and family level between treatment groups. Bacteroides were relatively abundant in the loperamide-induced slowed GI transit group, compared to controls. Richness and diversity of the bacterial communities was significantly lower in the loperamide-treated group compared to the control group. Understanding the link between specific microbial species and varying transit times is crucial to design interventions targeting the microbiome and to treat intestinal motility disorders.
dc.description.confidentialfalse
dc.format.pagination1139152-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/36998634
dc.identifier.citationParkar N, Dalziel JE, Spencer NJ, Janssen P, McNabb WC, Young W. (2023). Slowed gastrointestinal transit is associated with an altered caecal microbiota in an aged rat model.. Front Cell Infect Microbiol. 13. (pp. 1139152-).
dc.identifier.doi10.3389/fcimb.2023.1139152
dc.identifier.eissn2235-2988
dc.identifier.elements-typejournal-article
dc.identifier.issn2235-2988
dc.identifier.number1139152
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71766
dc.languageeng
dc.publisherFrontiers Media S.A.
dc.publisher.urihttps://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2023.1139152/full
dc.relation.isPartOfFront Cell Infect Microbiol
dc.rights(c) 2023 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectBacteroides
dc.subjectcaecal microbiota
dc.subjectenteric nervous system
dc.subjectintestinal motility
dc.subjectloperamide
dc.subjectRats
dc.subjectMale
dc.subjectAnimals
dc.subjectGastrointestinal Transit
dc.subjectLoperamide
dc.subjectRNA, Ribosomal, 16S
dc.subjectRats, Sprague-Dawley
dc.subjectConstipation
dc.subjectGastrointestinal Microbiome
dc.titleSlowed gastrointestinal transit is associated with an altered caecal microbiota in an aged rat model
dc.typeJournal article
pubs.elements-id460840
pubs.organisational-groupOther
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