UV-B Induced Flavonoids Contribute to Reduced Biotrophic Disease Susceptibility in Lettuce Seedlings.

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dc.citation.volume11
dc.contributor.authorMcLay ER
dc.contributor.authorPontaroli AC
dc.contributor.authorWargent JJ
dc.contributor.editorCarvalho SM
dc.coverage.spatialSwitzerland
dc.date.accessioned2024-11-05T22:18:54Z
dc.date.available2024-11-05T22:18:54Z
dc.date.issued2020-10-29
dc.description.abstractBiotrophic disease is one of the largest causes of decreased yield in agriculture. While exposure to ultraviolet B (UV-B) light (280-320 nm) has been previously observed to reduce plant susceptibility to disease, there is still a paucity of information regarding underlying biological mechanisms. In addition, recent advances in UV-LED technology raise the prospect of UV light treatments in agriculture which are practical and efficient. Here, we characterized the capability of UV-B LED pre-treatments to reduce susceptibility of a range of lettuce (Lactuca sativa) cultivars to downy mildew disease caused by the obligate biotroph Bremia lactucae. Innate cultivar susceptibility level did not seem to influence the benefit of a UV-B induced disease reduction with similar reductions as a percentage of the control observed (54-62% decrease in conidia count) across all susceptible cultivars. UV-B-induced reductions to conidia counts were sufficient to significantly reduce the infectivity of the diseased plant. Secondary infections caused by UV-B pre-treated plants exhibited yet further (67%) reduced disease severity. UV-B-induced flavonoids may in part mediate this reduced disease severity phenotype, as B. lactucae conidia counts of lettuce plants negatively correlated with flavonoid levels in a UV-B-dependent manner (r = -0.81). Liquid chromatography-mass spectrometry (LC-MS) was used to identify metabolic features which contribute to this correlation and, of these, quercetin 3-O-(6"-O-malonyl)-b-D-glucoside had the strongest negative correlation with B. lactucae conidia count (r = -0.68). When quercetin 3-O-(6"-O-malonyl)-b-D-glucoside was directly infiltrated into lettuce leaves, with those leaves subsequently infected, the B. lactucae conidia count was reduced (25-39%) in two susceptible lettuce cultivars. We conclude that UV-B induced phenolics, in particular quercetin flavonoids, may act as phytoanticipins to limit the establishment of biotrophic pathogens thus delaying or reducing their sporulation as measured by conidia count. These findings highlight the opportunity for UV-B morphogenesis to be exploited through the application of UV-LED technology, as part of the development of next-generation, sustainable disease control tools.
dc.description.confidentialfalse
dc.edition.edition2020
dc.format.pagination594681-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/33250915
dc.identifier.citationMcLay ER, Pontaroli AC, Wargent JJ. (2020). UV-B Induced Flavonoids Contribute to Reduced Biotrophic Disease Susceptibility in Lettuce Seedlings.. Front Plant Sci. 11. (pp. 594681-).
dc.identifier.doi10.3389/fpls.2020.594681
dc.identifier.eissn1664-462X
dc.identifier.elements-typejournal-article
dc.identifier.issn1664-462X
dc.identifier.numberARTN 594681
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71918
dc.languageeng
dc.publisherFrontiers Media S.A.
dc.publisher.urihttps://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.594681/full
dc.relation.isPartOfFront Plant Sci
dc.rights(c) 2020 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectBremia lactucae
dc.subjectLED
dc.subjectLactuca sativa
dc.subjectUV-B
dc.subjectdisease management
dc.subjectflavonoids
dc.subjectphotomorphogenesis
dc.titleUV-B Induced Flavonoids Contribute to Reduced Biotrophic Disease Susceptibility in Lettuce Seedlings.
dc.typeJournal article
pubs.elements-id436072
pubs.organisational-groupCollege of Health
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