Monoclonal antibodies indicate low-abundance links between heteroxylan and other glycans of plant cell walls

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dc.citation.issue6
dc.citation.volume242
dc.contributor.authorCornuault VRG
dc.contributor.authorBuffetto F
dc.contributor.authorRydahl MG
dc.contributor.authorMarcus SE
dc.contributor.authorTorode TA
dc.contributor.authorXue J
dc.contributor.authorCrepeau MJ
dc.contributor.authorFaria-Blanc N
dc.contributor.authorWillats WGT
dc.contributor.authorDupree P
dc.contributor.authorRalet MC
dc.contributor.authorKnox JP
dc.date.available25/07/2015
dc.date.issued25/07/2015
dc.description.abstractPlant cell walls are complex composites of structurally distinct glycans that are poorly understood in terms of both in muro inter-linkages and developmental functions. Monoclonal antibodies (MAbs) are versatile tools that can detect cell wall glycans with high sensitivity through the specific recognition of oligosaccharide structures. The isolation of two novel MAbs, LM27 and LM28, directed to heteroxylan, subsequent to immunisation with a potato cell wall fraction enriched in rhamnogalacturonan-I (RG-I) oligosaccharides, is described. LM27 binds strongly to heteroxylan preparations from grass cell walls and LM28 binds to a glucuronosyl-containing epitope widely present in heteroxylans. Evidence is presented suggesting that in potato tuber cell walls, some glucuronoxylan may be linked to pectic macromolecules. Evidence is also presented that suggests in oat spelt xylan both the LM27 and LM28 epitopes are linked to arabinogalactan-proteins as tracked by the LM2 arabinogalactan-protein epitope. This work extends knowledge of the potential occurrence of inter-glycan links within plant cell walls and describes molecular tools for the further analysis of such links.
dc.description.confidentialFALSE
dc.format.extent1321 - 1334
dc.identifier.citationPlanta, 2015, 242 (6), pp. 1321 - 1334
dc.identifier.elements-id403108
dc.identifier.harvestedMassey_Dark
dc.identifier.issn0032-0935
dc.identifier.urihttps://hdl.handle.net/10179/13066
dc.languageenglish
dc.publisherSpringer Verlag
dc.relation.isPartOfPlanta
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4605975/#__ffn_sectitle
dc.subjectarabinogalactan-protein
dc.subjectcell wall
dc.subjectglucuronoxylan
dc.subjectpectin
dc.subjectpolysaccharides
dc.subjectrhamnogalacturonan I
dc.subject.anzsrc0607 Plant Biology
dc.subject.anzsrc0703 Crop and Pasture Production
dc.titleMonoclonal antibodies indicate low-abundance links between heteroxylan and other glycans of plant cell walls
dc.typeJournal article
pubs.notesNot known
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Sciences
pubs.organisational-group/Massey University/College of Sciences/Institute of Fundamental Sciences
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