Journal Articles
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Item Agreement in Histological Assessment of Mitotic Activity Between Microscopy and Digital Whole Slide Images Informs Conversion for Clinical Diagnosis(SAGE Publications on behalf of the Association for Academic Pathology, 2022-03-28) Wei B-R; Halsey CH; Hoover SB; Puri M; Yang HH; Gallas BD; Lee MP; Chen W; Durham AC; Dwyer JE; Sánchez MD; Traslavina RP; Frank C; Bradley C; McGill LD; Esplin DG; Schaffer PA; Cramer SD; Lyle LT; Beck J; Buza E; Gong Q; Hewitt SM; Simpson RMValidating digital pathology as substitute for conventional microscopy in diagnosis remains a priority to assure effectiveness. Intermodality concordance studies typically focus on achieving the same diagnosis by digital display of whole slide images and conventional microscopy. Assessment of discrete histological features in whole slide images, such as mitotic figures, has not been thoroughly evaluated in diagnostic practice. To further gauge the interchangeability of conventional microscopy with digital display for primary diagnosis, 12 pathologists examined 113 canine naturally occurring mucosal melanomas exhibiting a wide range of mitotic activity. Design reflected diverse diagnostic settings and investigated independent location, interpretation, and enumeration of mitotic figures. Intermodality agreement was assessed employing conventional microscopy (CM40×), and whole slide image specimens scanned at 20× (WSI20×) and at 40× (WSI40×) objective magnifications. An aggregate 1647 mitotic figure count observations were available from conventional microscopy and whole slide images for comparison. The intraobserver concordance rate of paired observations was 0.785 to 0.801; interobserver rate was 0.784 to 0.794. Correlation coefficients between the 2 digital modes, and as compared to conventional microscopy, were similar and suggest noninferiority among modalities, including whole slide image acquired at lower 20× resolution. As mitotic figure counts serve for prognostic grading of several tumor types, including melanoma, 6 of 8 pathologists retrospectively predicted survival prognosis using whole slide images, compared to 9 of 10 by conventional microscopy, a first evaluation of whole slide image for mitotic figure prognostic grading. This study demonstrated agreement of replicate reads obtained across conventional microscopy and whole slide images. Hence, quantifying mitotic figures served as surrogate histological feature with which to further credential the interchangeability of whole slide images for primary diagnosis.Item Agreement in Histological Assessment of Mitotic Activity Between Microscopy and Digital Whole Slide Images Informs Conversion for Clinical Diagnosis(SAGE Publications on behalf of the Association for Academic Pathology, 2022-03-28) Wei B-R; Halsey CH; Hoover SB; Puri M; Yang HH; Gallas BD; Lee MP; Chen W; Durham AC; Dwyer JE; Sánchez MD; Traslavina RP; Frank C; Bradley C; McGill LD; Esplin DG; Schaffer PA; Cramer SD; Lyle LT; Beck J; Buza E; Gong Q; Hewitt SM; Simpson RMValidating digital pathology as substitute for conventional microscopy in diagnosis remains a priority to assure effectiveness. Intermodality concordance studies typically focus on achieving the same diagnosis by digital display of whole slide images and conventional microscopy. Assessment of discrete histological features in whole slide images, such as mitotic figures, has not been thoroughly evaluated in diagnostic practice. To further gauge the interchangeability of conventional microscopy with digital display for primary diagnosis, 12 pathologists examined 113 canine naturally occurring mucosal melanomas exhibiting a wide range of mitotic activity. Design reflected diverse diagnostic settings and investigated independent location, interpretation, and enumeration of mitotic figures. Intermodality agreement was assessed employing conventional microscopy (CM40×), and whole slide image specimens scanned at 20× (WSI20×) and at 40× (WSI40×) objective magnifications. An aggregate 1647 mitotic figure count observations were available from conventional microscopy and whole slide images for comparison. The intraobserver concordance rate of paired observations was 0.785 to 0.801; interobserver rate was 0.784 to 0.794. Correlation coefficients between the 2 digital modes, and as compared to conventional microscopy, were similar and suggest noninferiority among modalities, including whole slide image acquired at lower 20× resolution. As mitotic figure counts serve for prognostic grading of several tumor types, including melanoma, 6 of 8 pathologists retrospectively predicted survival prognosis using whole slide images, compared to 9 of 10 by conventional microscopy, a first evaluation of whole slide image for mitotic figure prognostic grading. This study demonstrated agreement of replicate reads obtained across conventional microscopy and whole slide images. Hence, quantifying mitotic figures served as surrogate histological feature with which to further credential the interchangeability of whole slide images for primary diagnosis.Item Co-production of insights for place-based approaches to revitalise te taiao in Aotearoa New Zealand(John Wiley and Sons Australia, Ltd on behalf of New Zealand Geographical Society, 2024-11-16) Turner JA; Stokes S; Jones RTH; Hemi M; Collins H; Vannier C; Burkitt L; Bradley C; Doehring K; Macintosh KA; Young J; Roskruge N; Perry-Smith P; Iosefa RK; Walker N; Young F; Bell A; McDermott A; Wood CCommunities across Aotearoa New Zealand are collaborating to reverse ecological decline, but little attention has been given to understanding the deeper relationship required with our physical and socio-cultural landscapes. We used knowledge co-production to develop 11 insights to support place-based strategies that nurture a collective responsibility to revitalise both people and place. Twenty-five subject matter experts across communities, government, industry and research drew from their collective expertise and the review of 63 local-to-global case study examples of farm-to-community-scale place-based approaches. A key output from this work is an Aotearoa New Zealand framework that diagrammatically represents the interdisciplinary nature of the 11 insights.
