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- ItemEffects of Green and Gold Kiwifruit Varieties on Antioxidant Neuroprotective Potential in Pigs as a Model for Human Adults.(MDPI (Basel, Switzerland), 2024-04-09) Kanon AP; Giezenaar C; Roy NC; Jayawardana IA; Lomiwes D; Montoya CA; McNabb WC; Henare SJ; Digiacomo MKiwifruit (KF) has shown neuroprotective potential in cell-based and rodent models by augmenting the capacity of endogenous antioxidant systems. This study aimed to determine whether KF consumption modulates the antioxidant capacity of plasma and brain tissue in growing pigs. Eighteen male pigs were divided equally into three groups: (1) bread, (2) bread + Actinidia deliciosa cv. 'Hayward' (green-fleshed), and (3) bread + A. chinensis cv. 'Hort16A' (yellow-fleshed). Following consumption of the diets for eight days, plasma and brain tissue (brain stem, corpus striatum, hippocampus, and prefrontal cortex) were collected and measured for biomarkers of antioxidant capacity, enzyme activity, and protein expression assessments. Green KF significantly increased ferric-reducing antioxidant potential (FRAP) in plasma and all brain regions compared with the bread-only diet. Gold KF increased plasma ascorbate concentration and trended towards reducing acetylcholinesterase activity in the brain compared with the bread-only diet. Pearson correlation analysis revealed a significant positive correlation between FRAP in the brain stem, prefrontal cortex, and hippocampus with the total polyphenol concentration of dietary interventions. These findings provide exploratory evidence for the benefits of KF constituents in augmenting the brain's antioxidant capacity that may support neurological homeostasis during oxidative stress.
- ItemThe use of confocal Raman microscopy and microfluidic channels to monitor the location and mobility of β-carotene incorporated in droplet-stabilized oil-in-water emulsions(Elsevier B V, 2023-05-17) Okubanjo SS; Brooke SJ; Ward R; Mostert N; Loveday SM; Ye A; Wilde PJ; Singh H; Waterland MThis study sought to explore the combined use of confocal Raman microscopy and microfluidic channels to probe the location and mobility of hydrophobic antioxidant (β-carotene) incorporated at the interface of food-grade droplet-stabilized emulsions (DSEs). Microfluidic channels were used to isolate emulsion droplets for efficient investigation of antioxidant mobility. This approach proved more conclusive than fixing the sample in agarose, because a single layer of droplets could be obtained. Results also indicated that the migration of β-carotene incorporated in shell droplets of olive oil and trimyristin DSEs to core droplets was minimal and beta-carotene remained mostly localised at the interface even after 3 days of production. This work demonstrates that microfluidic isolation of emulsion droplets combined with confocal Raman microscopy can give new insights into the spatial variation of chemical composition within emulsions. This study revealed that the migration of β-carotene between shell and core was minimal and hence it may be possible to concurrently deliver two incompatible compounds by spatially segregating them between shell and core compartments of DSEs.
- ItemFree-floating Planet Mass Function from MOA-II 9 yr Survey toward the Galactic Bulge(IOP Publishing, 2023-08-16) Sumi T; Koshimoto N; Bennett DP; Rattenbury NJ; Abe F; Barry R; Bhattacharya A; Bond IA; Fujii H; Fukui A; Hamada R; Hirao Y; Ishitani Silva S; Itow Y; Kirikawa R; Kondo I; Matsubara Y; Miyazaki S; Muraki Y; Olmschenk G; Ranc C; Satoh Y; Suzuki D; Tomoyoshi M; Tristram PJ; Vandorou A; Yama H; Yamashita KWe present the first measurement of the mass function of free-floating planets (FFPs), or very wide orbit planets down to an Earth mass, from the MOA-II microlensing survey in 2006-2014. Six events are likely to be due to planets with Einstein radius crossing times t E < 0.5 days, and the shortest has t E = 0.057 ± 0.016 days and an angular Einstein radius of θ E = 0.90 ± 0.14 μas. We measure the detection efficiency depending on both t E and θ E with image-level simulations for the first time. These short events are well modeled by a power-law mass function, dN 4/d log M = ( 2.18 − 1.40 + 0.52 ) × ( M / 8 M ⊕ ) − α 4 dex−1 star−1 with α 4 = 0.96 − 0.27 + 0.47 for M/M⊙ < 0.02. This implies a total of f = 21 − 13 + 23 FFPs or very wide orbit planets of mass 0.33 < M/M ⊕ < 6660 per star, with a total mass of 80 − 47 + 73 M ⊕ star−1. The number of FFPs is 19 − 13 + 23 times the number of planets in wide orbits (beyond the snow line), while the total masses are of the same order. This suggests that the FFPs have been ejected from bound planetary systems that may have had an initial mass function with a power-law index of α ∼ 0.9, which would imply a total mass of 171 − 52 + 80 M ⊕ star−1. This model predicts that Roman Space Telescope will detect 988 − 566 + 1848 FFPs with masses down to that of Mars (including 575 − 424 + 1733 with 0.1 ≤ M/M ⊕ ≤ 1). The Sumi et al. large Jupiter-mass FFP population is excluded.
- ItemConfirmation of Color-dependent Centroid Shift Measured After 1.8 Years with HST(American Astronomical Society, 2023-04-19) Bhattacharya A; Bennett DP; Beaulieu JP; Bond IA; Koshimoto N; Lu JR; Blackman JW; Ranc C; Vandorou A; Terry SK; Marquette JB; Cole AA; Fukui AWe measured the precise masses of the host and planet in the OGLE-2003-BLG-235 system, when the lens and source were resolving, with 2018 Keck high resolution images. This measurement is in agreement with the observation taken in 2005 with the Hubble Space Telescope (HST). In the 2005 data, the lens and sources were not resolved and the measurement was made using color-dependent centroid shift only. The Nancy Grace Roman Space Telescope will measure masses using data typically taken within 3-4 yr of the peak of the event, which is a much shorter baseline when compared to most of the mass measurements to date. Hence, the color-dependent centroid shift will be one of the primary methods of mass measurements for the Roman telescope. Yet, mass measurements of only two events (OGLE-2003-BLG-235 and OGLE-2005-BLG-071) have been done using the color-dependent centroid shift method so far. The accuracy of the measurements using this method are neither completely known nor well studied. The agreement of the Keck and HST results, as shown in this paper, is very important because this agreement confirms the accuracy of the mass measurements determined at a small lens-source separation using the color-dependent centroid shift method. It also shows that with >100 high resolution images, the Roman telescope will be able to use color-dependent centroid shift at a 3-4 yr time baseline and produce mass measurements. We find that OGLE-2003-BLG-235 is a planetary system that consists of a 2.34 ± 0.43M Jup planet orbiting a 0.56 ± 0.06M ⊙ K-dwarf host star at a distance of 5.26 ± 0.71 kpc from the Sun.
- ItemProtocol for the Gut Bugs in Autism Trial: a double-blind randomised placebo-controlled trial of faecal microbiome transfer for the treatment of gastrointestinal symptoms in autistic adolescents and adults.(BMJ Publishing Group, 2024-02-06) Tweedie-Cullen RY; Leong K; Wilson BC; Derraik JGB; Albert BB; Monk R; Vatanen T; Creagh C; Depczynski M; Edwards T; Beck K; Thabrew H; O'Sullivan JM; Cutfield WSINTRODUCTION: Autism (formally autism spectrum disorder) encompasses a group of complex neurodevelopmental conditions, characterised by differences in communication and social interactions. Co-occurring chronic gastrointestinal symptoms are common among autistic individuals and can adversely affect their quality of life. This study aims to evaluate the efficacy of oral encapsulated faecal microbiome transfer (FMT) in improving gastrointestinal symptoms and well-being among autistic adolescents and adults. METHODS AND ANALYSIS: This double-blind, randomised, placebo-controlled trial will recruit 100 autistic adolescents and adults aged 16-45 years, who have mild to severe gastrointestinal symptoms (Gastrointestinal Symptoms Rating Scale (GSRS) score ≥2.0). We will also recruit eight healthy donors aged 18-32 years, who will undergo extensive clinical screening. Recipients will be randomised 1:1 to receive FMT or placebo, stratified by biological sex. Capsules will be administered over two consecutive days following an overnight bowel cleanse with follow-up assessments at 6, 12 and 26 weeks post-treatment. The primary outcome is GSRS score at 6 weeks. Other assessments include anthropometry, body composition, hair cortisol concentration, gut microbiome profile, urine/plasma gut-derived metabolites, plasma markers of gut inflammation/permeability and questionnaires on general well-being, sleep quality, physical activity, food diversity and treatment tolerability. Adverse events will be recorded and reviewed by an independent data monitoring committee. ETHICS AND DISSEMINATION: Ethics approval for the study was granted by the Central Health and Disability Ethics Committee on 24 August 2021 (reference number: 21/CEN/211). Results will be published in peer-reviewed journals and presented to both scientific and consumer group audiences. TRIAL REGISTRATION NUMBER: ACTRN12622000015741.
- ItemThe Role of Gastric Lipase and Pepsin in Lipid Digestion of a Powder Infant Formula Using a Simulated Neonatal Gastric System(Springer Nature, 2024-02-08) Deng L; Golding M; Lentle R; MacGibbon A; Matia-Merino LThis study has sought to determine the impact of interfacial dynamics on the in vitro lipid digestion of a commercial infant formula; in particular, the specific role of interfacial proteolysis on the subsequent rates of reaction of droplet lipolysis. A powder infant formula was used as the as a protein-stabilised emulsion substrate during simulated infant gastric digestion at different pH level 3.5, 4.5 and 5.5. The digestate was treated with a fungal lipase and porcine pepsin (used to analogue human gastric lipase and pepsin) respectively and in a combined action. The study found that for fungal lipase treated digestate, the rate and extent of lipolysis were observed to be maxim at pH 5.5, in accordance with the optimal pH activity of the lipase. Findings also indicated that the proteinaceous interface did not appear to act as a barrier to lipolysis, since treatment with lipase and pepsin did not result in any significant increase in extent of lipolysis. However, it was observed that surface proteolysis did lead to alteration of the structural fate of the enzyme during digestion when compared to when the emulsion was digested solely by lipase. Findings suggest that lipolysis under these conditions may be independent of the structural dynamics of the emulsion during digestion, as observed within the context of this study design.
- ItemA Novel Casual Video Game With Simple Mental Health and Well-Being Concepts (Match Emoji): Mixed Methods Feasibility Study.(JMIR Publications, 2024-02-12) Pine R; Mbinta J; Te Morenga L; Fleming T; Leung TIBACKGROUND: Adolescence is a crucial phase for early intervention and prevention of mental health problems. Casual video games are popular and have promise as a novel mechanism for reaching young people, but this potential has seldom been explored. OBJECTIVE: This study aimed to explore the acceptability, feasibility, and possible indicators of therapeutic changes after playing a purpose-built novel casual video game (Match Emoji) with simple mental health and well-being content among young adolescents. METHODS: We conducted a single-arm, nonrandomized trial of Match Emoji with 12- to 14-year-old school students (N=45; 26 [57%] New Zealand European, 12 [26%] Māori; 7 [15%] Asian or Pacific; 27 [60%] boys, 3 [6%] non-binary). Participants were invited to play Match Emoji for 15 minutes, 2-3 times a week over 2 weeks (a total of 60 minutes). Acceptability was assessed through the frequency and duration of use (analytics analyzed at the end of the 2-week intervention period and at weeks 4 and 6) and through participant reports. The Child and Adolescent Mindfulness Measure (CAMM), General Help-Seeking Questionnaire (GHSQ), Flourishing Scale (FS), and Revised Children's Anxiety and Depression Scale (RCADS) were assessed at baseline and week 2 to indicate possible effects. Focus groups were held in week 4. RESULTS: Most participants (n=39, 87%) used Match Emoji for at least 60 minutes over the 2-week intervention, with 80% (36/45) continuing to play the game after the intervention period. Mean change (from baseline to 2 weeks) on each measure was 1.38 (95% CI -0.03 to 2.79; P=.06) for CAMM; 0.8 (95% CI -2.71 to 4.31; P=.64) for GHSQ; -1.09 (95% CI -2.83 to 0.66; P=.21) for FS; and -3.42 (95% CI -6.84 to -0.001; P=0.49) for RCADS. Focus group feedback suggested that Match Emoji was enjoyable and helpful. CONCLUSIONS: The casual video game with mental health content appeared to be acceptable and provided a promising indication of possible therapeutic effects. This approach is worthy of further investigation. INTERNATIONAL REGISTERED REPORT IDENTIFIER (IRRID): RR2-10.2196/31588.
- ItemFine-mapping analysis including over 254,000 East Asian and European descendants identifies 136 putative colorectal cancer susceptibility genes.(Springer Nature, 2024-04-26) Chen Z; Guo X; Tao R; Huyghe JR; Law PJ; Fernandez-Rozadilla C; Ping J; Jia G; Long J; Li C; Shen Q; Xie Y; Timofeeva MN; Thomas M; Schmit SL; Díez-Obrero V; Devall M; Moratalla-Navarro F; Fernandez-Tajes J; Palles C; Sherwood K; Briggs SEW; Svinti V; Donnelly K; Farrington SM; Blackmur J; Vaughan-Shaw PG; Shu X-O; Lu Y; Broderick P; Studd J; Harrison TA; Conti DV; Schumacher FR; Melas M; Rennert G; Obón-Santacana M; Martín-Sánchez V; Oh JH; Kim J; Jee SH; Jung KJ; Kweon S-S; Shin M-H; Shin A; Ahn Y-O; Kim D-H; Oze I; Wen W; Matsuo K; Matsuda K; Tanikawa C; Ren Z; Gao Y-T; Jia W-H; Hopper JL; Jenkins MA; Win AK; Pai RK; Figueiredo JC; Haile RW; Gallinger S; Woods MO; Newcomb PA; Duggan D; Cheadle JP; Kaplan R; Kerr R; Kerr D; Kirac I; Böhm J; Mecklin J-P; Jousilahti P; Knekt P; Aaltonen LA; Rissanen H; Pukkala E; Eriksson JG; Cajuso T; Hänninen U; Kondelin J; Palin K; Tanskanen T; Renkonen-Sinisalo L; Männistö S; Albanes D; Weinstein SJ; Ruiz-Narvaez E; Palmer JR; Buchanan DD; Platz EA; Visvanathan K; Ulrich CM; Siegel E; Brezina S; Gsur A; Campbell PT; Chang-Claude J; Hoffmeister M; Brenner H; Slattery ML; Potter JD; Tsilidis KK; Schulze MB; Gunter MJ; Murphy N; Castells A; Castellví-Bel S; Moreira L; Arndt V; Shcherbina A; Bishop DT; Giles GG; Southey MC; Idos GE; McDonnell KJ; Abu-Ful Z; Greenson JK; Shulman K; Lejbkowicz F; Offit K; Su Y-R; Steinfelder R; Keku TO; van Guelpen B; Hudson TJ; Hampel H; Pearlman R; Berndt SI; Hayes RB; Martinez ME; Thomas SS; Pharoah PDP; Larsson SC; Yen Y; Lenz H-J; White E; Li L; Doheny KF; Pugh E; Shelford T; Chan AT; Cruz-Correa M; Lindblom A; Hunter DJ; Joshi AD; Schafmayer C; Scacheri PC; Kundaje A; Schoen RE; Hampe J; Stadler ZK; Vodicka P; Vodickova L; Vymetalkova V; Edlund CK; Gauderman WJ; Shibata D; Toland A; Markowitz S; Kim A; Chanock SJ; van Duijnhoven F; Feskens EJM; Sakoda LC; Gago-Dominguez M; Wolk A; Pardini B; FitzGerald LM; Lee SC; Ogino S; Bien SA; Kooperberg C; Li CI; Lin Y; Prentice R; Qu C; Bézieau S; Yamaji T; Sawada N; Iwasaki M; Le Marchand L; Wu AH; Qu C; McNeil CE; Coetzee G; Hayward C; Deary IJ; Harris SE; Theodoratou E; Reid S; Walker M; Ooi LY; Lau KS; Zhao H; Hsu L; Cai Q; Dunlop MG; Gruber SB; Houlston RS; Moreno V; Casey G; Peters U; Tomlinson I; Zheng WGenome-wide association studies (GWAS) have identified more than 200 common genetic variants independently associated with colorectal cancer (CRC) risk, but the causal variants and target genes are mostly unknown. We sought to fine-map all known CRC risk loci using GWAS data from 100,204 cases and 154,587 controls of East Asian and European ancestry. Our stepwise conditional analyses revealed 238 independent association signals of CRC risk, each with a set of credible causal variants (CCVs), of which 28 signals had a single CCV. Our cis-eQTL/mQTL and colocalization analyses using colorectal tissue-specific transcriptome and methylome data separately from 1299 and 321 individuals, along with functional genomic investigation, uncovered 136 putative CRC susceptibility genes, including 56 genes not previously reported. Analyses of single-cell RNA-seq data from colorectal tissues revealed 17 putative CRC susceptibility genes with distinct expression patterns in specific cell types. Analyses of whole exome sequencing data provided additional support for several target genes identified in this study as CRC susceptibility genes. Enrichment analyses of the 136 genes uncover pathways not previously linked to CRC risk. Our study substantially expanded association signals for CRC and provided additional insight into the biological mechanisms underlying CRC development.
- ItemThe Behaviour of Sheep around a Natural Waterway and Impact on Water Quality during Winter in New Zealand(MDPI (Basel, Switzerland), 2023-04-25) Bunyaga A; Corner-Thomas R; Draganova I; Kenyon P; Burkitt L; Giuseppe PAccess of livestock, such as cattle, to waterways has been shown to be a cause of poor water quality due to pugging damage and excretion entering the water. In New Zealand, regulations require that cattle, deer, and pigs are excluded from accessing waterways, but there are no such requirements for sheep. The current study utilised 24 h video cameras, global positioning system units, and triaxial accelerometers to observe the interaction of Romney ewes (n = 40) with a natural waterway. Ewes were either restricted (week 1) or given access to a reticulated water trough (week 2). Proximity data showed that ewes spent more time within 3 m of the waterway when the trough was unrestricted than when restricted (14.1 ± 5.7 and 10.8 ± 5.1 min/ewe/day, respectively; p < 0.05). Ewes travelled shorter distances on the steeper areas of paddock than flatter areas. Similarly, ewes showed a spatial preference for the flat and low sloped areas of the paddock. Concentrations of suspended sediment and total phosphorus were higher during access to a reticulated water trough which coincided with the week with more rainy days. Phosphorus and E. coli concentrations in the stream water samples were the above recommended Australian and New Zealand Environment and Conservation Council water quality guidelines, especially after rainy days, but did not appear to be directly related to sheep activity. Overall, the results suggest that during winter, ewes interacted very little with the waterway and were thus unlikely to influence the levels of nutrient and pathogens in the waterway.
- ItemA new mapping tool to visualise critical infrastructure levels of service following a major earthquake(Elsevier B.V., 2024-01) Mowll R; Anderson MJ; Logan TM; Becker JS; Wotherspoon LM; Stewart C; Johnston D; Neely DHow can emergency management teams communicate to potentially impacted communities what a major event causing infrastructure outages might mean for them, and what they can do to prepare? In this paper we describe the process of creating a webtool for end users to visualise infrastructure outages that the Wellington region of New Zealand would face following a rupture of the Wellington fault. This webtool creates insight for three key groups: critical infrastructure owners, communities, and the emergency management sector itself. Critical infrastructure entities can use the tool to understand where they might consider infrastructure upgrades to mitigate gaps of delivery following a fault rupture, and to consider their emergency response plans for delivery in an emergency (leading to their consideration of ‘planning emergency levels of service’). Communities can use the tool to understand what infrastructure outages will mean at the household level in an emergency, including the considerable distances that some community members will have to walk to access services such as food and water and prepare for prolonged outages. Finally, with a greater knowledge of the gaps in delivery and of those community members that might need assistance with food and water collection, the emergency management sector can be better prepared. The methodology for creating the webtool is described, along with the insights that the completed webtool provides for emergency planning.
