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Item Validation of prediction equations to estimate the nutritive value of broiler chicken diets based on their chemical composition(Elsevier BV, Netherlands, 2025-02-18) Thiruchchenthuran S; Zaefarian F; Abdollahi MR; Wester TJ; Morel PCHAn experiment was conducted to validate the accuracy of previously published prediction equations developed to estimate the coefficient of apparent ileal digestibility (CAID) and ileal digestible content (IDC) of nitrogen (N), crude fat, starch, calcium (Ca), phosphorus (P), energy, and dry matter (DM) in broilers using the chemical composition of diets. Twenty new diets were formulated to have a wide range of chemical characteristics relevant to commercial diets. The CAID of N, crude fat, starch, Ca, P, energy, and DM of the diets were determined in broiler growers fed ad libitum from 15 to 22 days post-hatch. The chemical composition and in vivo digestibility values were used to validate the prediction equations developed from a previous study. Comparison between the determined values and predicted values was used to assess the accuracy of prediction equations using the coefficient of determination (R2), root mean square error of prediction, concordance correlation coefficient (CCC), and mean bias (MB). The most accurate prediction was achieved in terms of R2 and CCC for CAID of energy and DM (R2 = 0.57 and 0.66, CCC = 0.45 and 0.47, respectively) as well as for IDC of N, starch, energy, and DM (R2 = 0.90, 1.00, 0.65, and 0.66, CCC = 0.48, 0.97, 0.51, and 0.47, respectively). The R2 and CCC values obtained for CAID of N, crude fat, starch, Ca, and P and IDC of Ca and P were not consistent with the expectation of predictive performance. The R2 for IDC of crude fat was high (0.94), however, CCC was moderate (0.43). The determined MB values showed that some equations underpredicted (CAID and IDC of N, crude fat, starch, energy, and DM) and some overpredicted (CAID of Ca and P and IDC of P) the observed values of in vivo study. In conclusion, the equations obtained for CAID of energy and DM as well as IDC of N, starch, energy, and DM could be considered the best fit according to R2 and CCC. Moreover, this study highlights the importance of validation with external data before applying each prediction equation to practical situations.Item Effect of age on the standardized ileal amino acid digestibility of soybean meal and canola meal in broilers(Elsevier B.V. on behalf of KeAi Communications Co., Ltd., 2023-12-02) Barua M; Abdollahi MR; Zaefarian F; Wester TJ; Girish CK; Chrystal PV; Ravindran VStandardized ileal digestibility coefficients (SIDC) of nitrogen (N) and amino acids (AA) in two protein sources (soybean meal [SBM] and canola meal [CM]) were investigated at six broiler ages (d 7, 14, 21, 28, 35, and 42). Two assay diets were formulated to contain either SBM (413 g/kg) or CM (553 g/kg) as the sole dietary AA source. Titanium dioxide (5 g/kg) was added as an indigestible marker. A total of 696 male broilers at 1 d old were allotted to 12 replicate cages per age group. Each assay diet was offered to birds for 4 d prior to the ileal digesta collection on d 7 (14 birds/cage), 14 (12 birds/cage), 21 (10 birds/cage), 28 (8 birds/cage), 35 (8 birds/cage) and 42 (6 birds/cage), respectively. The apparent digestibility coefficients were standardized using age-specific basal endogenous AA flows. In the SBM group, though the SIDC of N tended to be influenced (quadratic; P = 0.075) by age, no linear or quadratic response of age effect was observed on the average SIDC of indispensable (IAA) and total AA (TAA). An age effect (quadratic; P < 0.05) was observed on the average SIDC of dispensable AA (DAA) in SBM with the highest value recorded at d 7, followed by a decrease from d 14 to 28, which increased beyond d 35. The SIDC of some individual AA (Arg, Thr, Trp, Cys, Pro) were affected (P < 0.05 or P < 0.001) in a quadratic manner by age. In the CM, the SIDC of N, average SIDC of IAA, DAA and TAA were influenced (quadratic; P < 0.05 or P < 0.001) by age. The SIDC of N and average SIDC of DAA and TAA were higher from d 7 to 14, declined at d 21, and then increased beyond d 28. The average SIDC of IAA was low between d 7 and 28 and increased thereafter. The SIDC of individual AA were affected (linear or quadratic; P < 0.05 or P < 0.001) by different magnitudes by age. The age influence on the SIDC AA was variable, depending on the protein source and AA. The results demonstrate that age-specific SIDC AA data might need consideration in broiler feed formulations.
