Journal Articles
Permanent URI for this collectionhttps://mro.massey.ac.nz/handle/10179/7915
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Item Intra-molecular lysine-arginine derived advanced glycation end-product cross-linking in Type I collagen: A molecular dynamics simulation study.(2016-11) Collier TA; Nash A; Birch HL; de Leeuw NHCovalently cross-linked advanced glycation end products (AGE) are among the major post-translational modifications to proteins as a result of non-enzymatic glycation. The formation of AGEs has been shown to have adverse effects on the properties of the collagenous tissue; they are even linked to a number of age related disorders. Little is known about the sites at which these AGEs form or why certain sites within the collagen are energetically more favourable than others. In this study we have used a proven fully atomistic molecular dynamics approach to identify six sites where the formation of the intra-molecular 3-deoxyglucosone-derived imidazolium cross-link (DOGDIC) is energetically favourable. We have also conducted a comparison of these positions with those of the more abundant glucosepane cross-link, to determine any site preference. We show that when we consider both lysine and arginine AGEs, they exhibit a prevalence to form within the gap region of the collagen fibril.Item The Prospect for an Australian–Asian Power Grid: A Critical Appraisal(MDPI AG, 15/01/2018) Halawa E; Shi X; Nepal R; Sari NH; James GAustralia is an energy net self-sufficient country rich in energy resources, from fossil-based to renewable energy. Australia, a huge continent with low population density, has witnessed impressive reduction in energy consumption in various sectors of activity in recent years. Currently, coal and natural gas are two of Australia's major export earners, yet its abundant renewable energy resources such as solar, wind, and tidal, are still underutilized. The majority of Asian countries, on the other hand, are in the middle of economic expansion, with increasing energy consumption and lack of energy resources or lack of energy exploration capability becoming a serious challenge. Electricity interconnection linking two or more independent grids within a country or at cross-border or regional levels has found its way into electricity markets worldwide. This concept allows for electricity exchanges that lead to optimized use and sharing of electricity generated from different sources. The interconnection also enables the long distance exploitation of renewable energy which would otherwise be physically impossible. ASEAN (Association of Southeast Asian Nations) and other regional groupings in Asia have initiated a number of interconnections to gain economic benefits. Asian's hunger for energy for its economic development, climate change that has become a global and urgent issue to be solved, and Australia's abundant renewable energy resources have all prompted increasing interest in a super-grid interconnection linking Australia to Asian grids, the Australian-Asian (Power) Grid (AAG). This paper overviews the existing grid interconnections as well as current initiatives at domestic, sub-regional, and regional levels worldwide, with a particular focus on Asia. The paper concludes with a critical appraisal on the benefits, potential, challenges and issues to be encountered by the AAG initiative.Item Single-molecule folding mechanisms of the apo- and Mg(2+)-bound states of human neuronal calcium sensor-1(Biophysical Society, 7/07/2015) Naqvi MM; Heidarsson PO; Rodriguez Otazo M; Mossa A; Kragelund BB; Cecconi CNeuronal calcium sensor-1 (NCS-1) is the primordial member of a family of proteins responsible primarily for sensing changes in neuronal Ca(2+) concentration. NCS-1 is a multispecific protein interacting with a number of binding partners in both calcium-dependent and independent manners, and acting in a variety of cellular processes in which it has been linked to a number of disorders such as schizophrenia and autism. Despite extensive studies on the Ca(2+)-activated state of NCS proteins, little is known about the conformational dynamics of the Mg(2+)-bound and apo states, both of which are populated, at least transiently, at resting Ca(2+) conditions. Here, we used optical tweezers to study the folding behavior of individual NCS-1 molecules in the presence of Mg(2+) and in the absence of divalent ions. Under tension, the Mg(2+)-bound state of NCS-1 unfolds and refolds in a three-state process by populating one intermediate state consisting of a folded C-domain and an unfolded N-domain. The interconversion at equilibrium between the different molecular states populated by NCS-1 was monitored in real time through constant-force measurements and the energy landscapes underlying the observed transitions were reconstructed through hidden Markov model analysis. Unlike what has been observed with the Ca(2+)-bound state, the presence of Mg(2+) allows both the N- and C-domain to fold through all-or-none transitions with similar refolding rates. In the absence of divalent ions, NCS-1 unfolds and refolds reversibly in a two-state reaction involving only the C-domain, whereas the N-domain has no detectable transitions. Overall, the results allowed us to trace the progression of NCS-1 folding along its energy landscapes and provided a solid platform for understanding the conformational dynamics of similar EF-hand proteins.
