IKVAV-Containing Cell Membrane Penetrating Peptide Treatment Induces Changes in Cellular Morphology after Spinal Cord Injury
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Date
2016-08-17
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Publisher
Canadian Center of Science and Education
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Abstract
A cell membrane spanning peptide was used to increase the concentration of the IKVAV motif within damaged
mouse spinal cord tissue. This peptide was injected directly to the lesion 24 hours after spinal cord compression
injury. Because the membrane-spanning portion of the peptide adheres to tissue upon injection with a long
half-life we hypothesized that the bioactive IKVAV sequence will provide a sustained regenerative signal at the
sight of injury. Five different groups of mice were used and cellular morphology observations were undertaken
using light and electron microscopy. Three surgical control groups: IKVAV, peptide and mannitol; one surgical
treatment group: IKVAV-peptide; and one non-surgical control group: normal, were used in this experiment. In
this study, treatment with IKVAV-peptide after SCI resulted in an increased number of protoplasmic astrocytes,
large active motor neurons, and regeneration of muscle bundles followed by behavioral improvement. In this
paper, we describe the cellular differences between all groups.
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Keywords
IKVAV+peptide, neurons, protoplasmic astrocytes, muscle bundles, light micrographs, electron micrographs
Citation
Modern Applied Science, 2016, 10 (11)