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dc.contributor.authorPalmer, Ren_US
dc.contributor.authorCornuault, VRGen_US
dc.contributor.authorMarcus, SEen_US
dc.contributor.authorKnox, JPen_US
dc.contributor.authorShewry, PRen_US
dc.contributor.authorTosi, Pen_US
dc.date.available2015-03-01en_US
dc.date.issued2015-03-01en_US
dc.identifier.citationPlanta, 2015, 241 (3), pp. 669 - 685en_US
dc.identifier.issn0032-0935en_US
dc.description.abstractCell wall polysaccharides of wheat and rice endosperm are an important source of dietary fibre. Monoclonal antibodies specific to cell wall polysaccharides were used to determine polysaccharide dynamics during the development of both wheat and rice grain. Wheat and rice grain present near synchronous developmental processes and significantly different endosperm cell wall compositions, allowing the localisation of these polysaccharides to be related to developmental changes. Arabinoxylan (AX) and mixed-linkage glucan (MLG) have analogous cellular locations in both species, with deposition of AX and MLG coinciding with the start of grain filling. A glucuronoxylan (GUX) epitope was detected in rice, but not wheat endosperm cell walls. Callose has been reported to be associated with the formation of cell wall outgrowths during endosperm cellularisation and xyloglucan is here shown to be a component of these anticlinal extensions, occurring transiently in both species. Pectic homogalacturonan (HG) was abundant in cell walls of maternal tissues of wheat and rice grain, but only detected in endosperm cell walls of rice in an unesterified HG form. A rhamnogalacturonan-I (RG-I) backbone epitope was observed to be temporally regulated in both species, detected in endosperm cell walls from 12 DAA in rice and 20 DAA in wheat grain. Detection of the LM5 galactan epitope showed a clear distinction between wheat and rice, being detected at the earliest stages of development in rice endosperm cell walls, but not detected in wheat endosperm cell walls, only in maternal tissues. In contrast, the LM6 arabinan epitope was detected in both species around 8 DAA and was transient in wheat grain, but persisted in rice until maturity.en_US
dc.format.extent669 - 685en_US
dc.languageEnglishen_US
dc.publisherSpringer Verlagen_US
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4328131/#__ffn_sectitleen_US
dc.rights© The Author(s) 2014 Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are crediteden_US
dc.subjectcell wallen_US
dc.subjectgrain developmenten_US
dc.subjectimmunodetectionen_US
dc.subjectrice grainen_US
dc.subjectwheat grainen_US
dc.titleComparative in situ analyses of cell wall matrix polysaccharide dynamics in developing rice and wheat grainen_US
dc.typeJournal Article
dc.citation.volume241en_US
dc.identifier.doi10.1007/s00425-014-2201-4en_US
dc.description.confidentialfalseen_US
dc.identifier.elements-id403211
dc.relation.isPartOfPlantaen_US
dc.citation.issue3en_US
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Sciences
pubs.organisational-group/Massey University/College of Sciences/Institute of Fundamental Sciences
dc.identifier.harvestedMassey_Dark
pubs.notesNot knownen_US
dc.subject.anzsrc0607 Plant Biologyen_US
dc.subject.anzsrc0703 Crop And Pasture Productionen_US


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