The lipid metabolism of plants : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Biochemistry at Massey University
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Date
1968
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Massey University
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Abstract
A method , based on the isolation of pure compounds
by a combination of DEAE-cellulose and thin-layer
chromatography , has been developed for the rapid and
quantitative estimation of the major glycerolipids of
plant tissues. The method has been used 1) for the
analyses of the major glycerolipids of a wide variety
of plant species and 2) as part of a detailed chromatographic analysis of the glycerolipid constituents of
the green alga Mesotaenium caldariorurn during which
two unknowm lipids, probably glycerolipids, were
isolated.
On the basis of the incorporation of radiocarbon
from 14C-labelled precursors into the glycerlipids of both Mesotaenium cells and pumpkin leaves, the likelyhood [sic] of relatively low turnover rates for the various glycerolipids, with the possible exceptions of phosphatidyl glycerol in Mesotaenium and phosphatidyl choline in pumpkin leaf, is discussed.
The unusual growth requirements of Mesotaenium caldariorum in liquid culture is discussed briefly.
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Keywords
Plant metabolism, Lipids, Mesotaenium caldariorum