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dc.contributor.authorde Vegt, Piet
dc.date.accessioned2015-08-09T23:49:58Z
dc.date.available2015-08-09T23:49:58Z
dc.date.issued2015
dc.identifier.urihttp://hdl.handle.net/10179/6954
dc.description.abstractMilk is an excellent food source as it contains a plentiful supply of nutrients and minerals. Although normally consumed after pasteurisation, there is growing evidence that raw milk provides health benefits beyond nutrition alone. Epidemiological studies of children have shown that those who regularly consume raw milk appear to have a lower incidence of asthma and non-­-specific allergy than those who consume processed commercial milk. The gastrointestinal tract is a key location for immune development as interaction with microflora can occur at the mucosal surface. Milk may have a role to play in the early stages of this process either due to the microbes it harbours or to the physical and chemical properties of milk itself. The aim of this study was to identify bacterial isolates unique to raw milk, that would not survive pasteurisation; and to determine whether milk allowed for a greater survival of these bacteria during ingestion. Bacterial isolates were cultured from either raw or pasteurised milk and tested for their ability to survive pasteurisation. A subset of thermosensitive isolates were identified for further analysis representing those species likely to be present in raw milk but not processed. This thermosensitive subset was challenged for their ability to tolerate acid conditions (pH 2.5) both in the presence and absence of milk to determine the likelihood of their survival during ingestion. A high throughput acid tolerance test was developed to screen raw milk bacteria for acid tolerance. Data supports the hypothesis that milk significantly increased the survival of raw milk bacteria exposed to pH 2.5 and that specific components found specifically in milk were, at least in part responsible for this effect. In conclusion, a unique subset of bacteria found only in raw bovine milk, and not in processed milk, has been identified that when ingested with milk are able to come through an acid challenge not dissimilar to that of the stomach and survive. This opens the possibility that these bacteria present in raw milk are able to enter the lower GI tract and interact with the immune system via Peyers patches.en_US
dc.language.isoenen_US
dc.publisherMassey Universityen_US
dc.rightsThe Authoren_US
dc.subjectRaw milken_US
dc.subjectMicrobiologyen_US
dc.subjectHealth aspectsen_US
dc.subjectGastrointestinal systemen_US
dc.subjectLow pHen_US
dc.subjectAcid toleranceen_US
dc.titleThe microflora of raw milk and the impact of milk on their survival at low pH : a thesis presented in partial fulfilment of the requirements for the degree of Doctorate of Philosophy in Food Technology at Massey University, Palmerston North, New Zealanden_US
dc.typeThesisen_US
thesis.degree.disciplineFood Technologyen_US
thesis.degree.grantorMassey Universityen_US
thesis.degree.levelDoctoralen_US
thesis.degree.nameDoctor of Philosophy (Ph.D.)en_US


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