In Vitro Ileal Fermentation is Affected More by the Fiber Source Fermented than the Ileal Microbial Composition in Growing Pigs

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dc.citation.issue5
dc.citation.volume7
dc.contributor.authorHoogeveen AM
dc.contributor.authorMoughan PJ
dc.contributor.authorHodgkinson SM
dc.contributor.authorStroebinger N
dc.contributor.authorYu W
dc.contributor.authorRettedal EA
dc.contributor.authorMcNabb WC
dc.contributor.authorMontoya CA
dc.coverage.spatialUnited States
dc.date.accessioned2024-10-14T01:41:27Z
dc.date.available2024-10-14T01:41:27Z
dc.date.issued2023-05
dc.description.abstractBACKGROUND: The fermentation of undigested material in the ileum is quantitatively important. However, the respective contributions of the microbial composition and the substrate to ileal fermentation are unclear. OBJECTIVE: This aim was to investigate the contribution of microbial composition and fiber source to in vitro ileal fermentation outcomes. METHODS: Thirteen ileal cannulated female pigs (Landrace/Large White; 9-wk-old; 30.5 kg body weight) were given diets containing black beans, wheat bread, chickpeas, peanuts, pigeon peas, sorghum, or wheat bran as the sole protein source for 7 d (100 g protein/kg dry matter diet). On day 7, ileal digesta were collected and stored at -80°C for microbial analysis and in vitro fermentation. For each diet, a pooled ileal inoculum was prepared to ferment different fiber sources (cellulose, pectin, arabinogalactan, inulin, fructooligosaccharides, and resistant starch) for 2 h at 37°C. Organic matter fermentability and organic acid production were determined following in vitro fermentation. Data were analyzed using a 2-way ANOVA (inoculum × fiber). RESULTS: Forty-five percent of the identified genera in the digesta differed across diets. For instance, the number of Lactococcus was 115-fold greater (P ≤ 0.05) in the digesta of pigs fed the pigeon pea diet than for pigs fed the wheat bran diet. For both in vitro organic matter fermentability and organic acid production, there were significant (P ≤ 0.05) interactions between the inoculum and the fiber source. For instance, pectin and resistant starch resulted in 1.6- to 31-fold more (P ≤ 0.05) lactic acid production when fermented by the pigeon pea inoculum than other inocula. For specific fiber sources, statistically significant correlations were found between the number of bacteria from certain members of the ileal microbial community and fermentation outcomes. CONCLUSIONS: Both the fiber source fermented and the ileal microbial composition of the growing pig affected in vitro fermentation; however, the effect of the fiber source was predominant.Curr Dev Nutr 2023;x:xx.
dc.description.confidentialfalse
dc.edition.editionMay 2023
dc.format.pagination100076-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/37180852
dc.identifier.citationHoogeveen AM, Moughan PJ, Hodgkinson SM, Stroebinger N, Yu W, Rettedal EA, McNabb WC, Montoya CA. (2023). In Vitro Ileal Fermentation is Affected More by the Fiber Source Fermented than the Ileal Microbial Composition in Growing Pigs.. Curr Dev Nutr. 7. 5. (pp. 100076-).
dc.identifier.doi10.1016/j.cdnut.2023.100076
dc.identifier.eissn2475-2991
dc.identifier.elements-typejournal-article
dc.identifier.issn2475-2991
dc.identifier.number100076
dc.identifier.piiS2475-2991(23)21249-4
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71700
dc.languageeng
dc.publisherElsevier Inc. on behalf of American Society for Nutrition
dc.publisher.urihttps://www.sciencedirect.com/science/article/pii/S2475299123212494
dc.relation.isPartOfCurr Dev Nutr
dc.subjectdietary fiber
dc.subjecthuman
dc.subjectileal microbiota
dc.subjectin vitro ileal fermentation
dc.subjectpig
dc.titleIn Vitro Ileal Fermentation is Affected More by the Fiber Source Fermented than the Ileal Microbial Composition in Growing Pigs
dc.typeJournal article
pubs.elements-id461296
pubs.organisational-groupOther
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