Towards Gut Microbiota Sampling Using an Untethered Sampling Device

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dc.citation.volume9
dc.contributor.authorRehan M
dc.contributor.authorAl-Bahadly I
dc.contributor.authorThomas DG
dc.contributor.authorAvci E
dc.date.accessioned2024-01-18T22:49:36Z
dc.date.accessioned2024-07-25T06:32:24Z
dc.date.available2024-01-18T22:49:36Z
dc.date.available2024-07-25T06:32:24Z
dc.date.issued2021-09-09
dc.description.abstractRecent studies suggest that human gut microbiota can act as a bio-marker for human health. Also, it can function as a potential tool to understand stress and anxiety. However, the conventional tools have limitations acquiring samples of gut microbiota without contamination. In this work, an untethered robotic capsule prototype is developed that can actively collect the microbiota from the mucosa layer of the small intestine for the first time with the potential to avoid the upstream and downstream contamination. An analytical model for quantifying the peristaltic forces and developing two-way shape memory alloy spring actuator is presented. For the first time, a novel two-way shape memory alloy spring actuator (5 mm x \phi ~4 mm) is used to perform the sampling inside the gut. The spring actuator can apply 675 mN force, which is sufficient to perform in vivo sampling. A specialised experimental setup that can keep the freshly dissected intestine alive for 6 hours is utilised to test the robotic capsule. The robotic capsule prototype has collected an average of 200~\mu L and 112~\mu L sample from living pig duodenal and ileal tissues respectively i.e. in the presence of peristaltic forces. The robotic capsule was also tested on intestine of other species including cow and sheep and collected an average of 160~\mu L and 185~\mu L of content respectively from the living post-mortem tissues. The collected sample size for all the species is feasible to analyse the microbiota through next generation sequencing techniques. The experimental setup is a reliable proxy to in-vivo behaviour and the robotic capsule experimental result is promising in terms of in situ collection of microbiota.
dc.format.pagination127175-127184
dc.identifier.author-urlhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000696666600001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=c5bb3b2499afac691c2e3c1a83ef6fef
dc.identifier.citationRehan M, Al-Bahadly I, Thomas DG, Avci E. (2021). Towards Gut Microbiota Sampling Using an Untethered Sampling Device. IEEE Access. 9. (pp. 127175-127184).
dc.identifier.doi10.1109/ACCESS.2021.3111086
dc.identifier.eissn2169-3536
dc.identifier.elements-typejournal-article
dc.identifier.issn2169-3536
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/70379
dc.publisherIEEE
dc.relation.isPartOfIEEE Access
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectCapsule endoscopy
dc.subjectGI tract
dc.subjectperistaltic motion
dc.subjectrobotic capsule
dc.subjectshape memory alloy spring actuator
dc.titleTowards Gut Microbiota Sampling Using an Untethered Sampling Device
dc.typeJournal article
pubs.elements-id448492
pubs.organisational-groupOther
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