The aetiology of wobbly possum disease: Reproduction of the disease with purified nidovirus.
Loading...
![Thumbnail Image](assets/images/mime.png)
Date
2016-04
Open Access Location
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Rights
Abstract
The objective of this study was to investigate a role of a recently discovered marsupial nidovirus in the development of a neurological disease, termed wobbly possum disease (WPD), in the Australian brushtail possum (Trichosurus vulpecula). Four possums received 1 mL of a standard inoculum that had been prepared from tissues of WPD-affected possums, 4 possums received 1.8 mL (1 × 10(6) TCID50) of a cell lysate from inoculated cultures, and 4 possums received 1 mL (× 10(7) TCID50) of a purified WPD isolate. All but one possum that received infectious inocula developed neurological disease and histopathological lesions characteristic for WPD. High levels of viral RNA were detected in livers from all possums that received infectious inocula, but not from control possums. Altogether, our data provide strong experimental evidence for the causative involvement of WPD virus in development of a neurological disease in infected animals.
Description
Keywords
Arterivirus, Wobbly possum disease, WPD, Australian brushtail possum, Trichosurus vulpecula, Koch's postulates, Aetiology
Citation
VIROLOGY, 2016, 491 pp. 20 - 26