Effects of extremely low frequency electromagnetic fields on human chromosomes : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Genetics at the Institute of Molecular BioSciences, Massey University, Palmerston North, New Zealand

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dc.contributor.authorWahab, Mohammed Abdul
dc.date.accessioned2010-08-23T21:42:06Z
dc.date.availableNO_RESTRICTIONen_US
dc.date.available2010-08-23T21:42:06Z
dc.date.issued2005
dc.description.abstractElectromagnetic fields (EMFs) have been associated with increased incidences of cancer as suggested by epidemiological studies. The in vitro sister chromatid exchange (SCE) technique, radiation-induced micronucleus assay (MN assay), COMET assay, and fluorescence in situ hybridization (FISH) were used in the present study to test the carcinogenic potentiality of extremely low frequency (ELF) EMFs on human peripheral blood lymphocytes. All experiments were performed single blind and used lymphocytes taken from 6 age-matched donors. The SCE experiments were conducted twice: round 1 (R1) and round 2 (R2), in order to determine whether or not the results obtained could be duplicated. Detailed analysis of the SCE results showed that there was a significant increase in the number of SCEs/cell in the grouped experimental conditions compared to the controls in both rounds. Similarly, in the MN assay, a significant increase of mean number of micronucleated CB cells/100 CB cells (Ma) and mean number of micronuclei/100 CB cells (Mb) was observed in the grouped experimental conditions compared to the controls. Moreover, the highest SCE frequency in R1 was 10.03 for a square continuous field, and the SCE frequency of 10.39 for a square continuous field in R2 (albeit a different strength) was the second highest in this latter round. But in the MN assay a square pulsed field with increasing EMF strength showed the greatest effect on the DNA repair system. The COMET assay also showed that both a l m T square field (continuous or pulsed) resulted in significant fragmentation of the DNA. On the other hand, a FISH analysis failed to show any translocations. In the field of EMF research, perhaps the most outstanding question that remains to be answered with certainty is how weak EMFs exert their effects at the molecular level. Various mechanisms are reviewed and evaluated in this thesis. From the results of the research performed in the current study which concentrated on testing and discovering genetic effects, a model is postulated that weak EMFs stimulate the production of free radicals which result in genetic damage. Further extensive research should be conducted to test this hypothesis.en_US
dc.identifier.urihttp://hdl.handle.net/10179/1582
dc.language.isoenen_US
dc.publisherMassey Universityen_US
dc.rightsThe Authoren_US
dc.subjectElectromagnetic fields and canceren_US
dc.subjectEffects of electromagnetic fieldsen_US
dc.subjectGenetic damageen_US
dc.subject.otherFields of Research::270000 Biological Sciences::270200 Geneticsen_US
dc.titleEffects of extremely low frequency electromagnetic fields on human chromosomes : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Genetics at the Institute of Molecular BioSciences, Massey University, Palmerston North, New Zealanden_US
dc.typeThesisen_US
massey.contributor.authorWahab, Mohammed Abdul
thesis.degree.disciplineGeneticsen_US
thesis.degree.grantorMassey Universityen_US
thesis.degree.levelDoctoralen_US
thesis.degree.levelDoctoralen
thesis.degree.nameDoctor of Philosophy (Ph.D.)en_US
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