Influence of Operating Conditions on Reuterin Production Using Resting Cells of Limosilactobacillus reuteri DPC16

Simple item page
dc.citation.issue5
dc.citation.volume8
dc.contributor.authorSun Y
dc.contributor.authorGutierrez-Maddox N
dc.contributor.authorMutukumira AN
dc.contributor.authorMaddox IS
dc.contributor.authorShu Q
dc.contributor.editorRaganati F
dc.contributor.editorProcentese A
dc.date.accessioned2023-10-24T02:11:03Z
dc.date.accessioned2023-10-25T20:49:40Z
dc.date.available2022-05-16
dc.date.available2023-10-24T02:11:03Z
dc.date.available2023-10-25T20:49:40Z
dc.date.issued2022-05-16
dc.date.updated2023-10-24T01:47:51Z
dc.description.abstractLimosilactobacillus reuteri strains can secrete a potentially valuable chemical and broadspectrum antimicrobial substance named reuterin (3-hydroxypropionaldehyde, 3-HPA). L. reuteri DPC16 is a novel and patented probiotic strain that is used commercially because of its proven ability to kill various foodborne pathogens. A two-step process has been developed for reuterin production from glycerol using L. reuteri DPC16. Cells were grown, followed by harvesting, and then were incubated with glycerol for reuterin production. Parameters investigated during the glycerol conversion included the initial glycerol concentration, the biomass concentration, pH, culture age at harvesting, conversion time, and temperature. The highest reuterin yield was obtained using 21 g/L 24 h old cells, to convert glycerol solution (300 mmol/L) in 1 h at 30◦ C and pH 6.2. The most efficient transformation of glycerol to reuterin was achieved in approximately 20 h of growth of cells at 25◦ C and pH 6.8. Using the regression equation of this study, the maximum concentration of reuterin can be obtained using 25 g/L 20 h old DPC6 cells to ferment 350 mmol/L glycerol (initial concentration) for 2 h at 25◦ C and pH 6.8 The ranking of effects on reuterin production for the six single factors was glycerol concentration > pH > conversion time > biomass concentration > temperature > culture age.
dc.description.confidentialfalse
dc.edition.editionMay 2022
dc.identifier227
dc.identifier.citationSun Y, Gutierrez-Maddox N, Mutukumira AN, Maddox IS, Shu Q. (2022). Influence of Operating Conditions on Reuterin Production Using Resting Cells of Limosilactobacillus reuteri DPC16. Fermentation. 8. 5.
dc.identifier.doi10.3390/fermentation8050227
dc.identifier.eissn2311-5637
dc.identifier.elements-typejournal-article
dc.identifier.harvestedMassey_Dark
dc.identifier.urihttp://hdl.handle.net/10179/20404
dc.languageEnglish
dc.publisherMDPI (Basel, Switzerland)
dc.publisher.urihttps://www.mdpi.com/2311-5637/8/5/227
dc.relation.isPartOfFermentation
dc.rights(c) 2022 The Author/sen_US
dc.rightsCC BYen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subjectLimosilactobacillus reuteri DPC16
dc.subjectreuterin
dc.subject3-hydroxypropionaldehyde
dc.subjectbioconversion
dc.subjectprobiotics
dc.titleInfluence of Operating Conditions on Reuterin Production Using Resting Cells of Limosilactobacillus reuteri DPC16
dc.typeJournal article
pubs.elements-id453609
pubs.organisational-groupOther
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
453609 PDF.pdf
Size:
3.16 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Journal Articles