Extensive bacteriocin gene shuffling in the Streptococcus bovis/Streptococcus equinus complex reveals gallocin D with activity against vancomycin resistant enterococci.
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Date
2020-08-10
Open Access Location
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Publisher
Springer Nature Limited
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(c) 2020 The Author/s
CC BY 4.0
CC BY 4.0
Abstract
Streptococcus gallolyticus LL009 produces gallocin D, a narrow spectrum two component bacteriocin with potent activity against vancomycin-resistant enterococci. Gallocin D is distinct from gallocin A, a separate two component bacteriocin produced by S. gallolyticus. Although the gene clusters encoding gallocin A and gallocin D have a high degree of gene synteny, the structural genes are highly variable and appear to have undergone gene shuffling with other streptococcal species. Gallocin D was analysed in laboratory-based experiments. The mature peptides are 3,343 ± 1 Da and 3,019 ± 1 Da and could be readily synthesized and display activity against a vancomycin resistant Enterococcus strain EC300 with a MIC value of 1.56 µM. Importantly, these bacteriocins could contribute to the ability of S. gallolyticus to colonize the colon where they have been associated with colorectal cancer.
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Keywords
Amino Acid Sequence, Bacteriocins, Computer Simulation, Drug Resistance, Bacterial, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Streptococcal Infections, Streptococcus, Streptococcus bovis, Vancomycin, Vancomycin-Resistant Enterococci
Citation
Hill D, O'Connor PM, Altermann E, Day L, Hill C, Stanton C, Ross RP. (2020). Extensive bacteriocin gene shuffling in the Streptococcus bovis/Streptococcus equinus complex reveals gallocin D with activity against vancomycin resistant enterococci.. Sci Rep. 10. 1. (pp. 13431-).