Extensive bacteriocin gene shuffling in the Streptococcus bovis/Streptococcus equinus complex reveals gallocin D with activity against vancomycin resistant enterococci.

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dc.citation.issue1
dc.citation.volume10
dc.contributor.authorHill D
dc.contributor.authorO'Connor PM
dc.contributor.authorAltermann E
dc.contributor.authorDay L
dc.contributor.authorHill C
dc.contributor.authorStanton C
dc.contributor.authorRoss RP
dc.coverage.spatialEngland
dc.date.accessioned2024-08-21T02:58:52Z
dc.date.available2024-08-21T02:58:52Z
dc.date.issued2020-08-10
dc.description.abstractStreptococcus gallolyticus LL009 produces gallocin D, a narrow spectrum two component bacteriocin with potent activity against vancomycin-resistant enterococci. Gallocin D is distinct from gallocin A, a separate two component bacteriocin produced by S. gallolyticus. Although the gene clusters encoding gallocin A and gallocin D have a high degree of gene synteny, the structural genes are highly variable and appear to have undergone gene shuffling with other streptococcal species. Gallocin D was analysed in laboratory-based experiments. The mature peptides are 3,343 ± 1 Da and 3,019 ± 1 Da and could be readily synthesized and display activity against a vancomycin resistant Enterococcus strain EC300 with a MIC value of 1.56 µM. Importantly, these bacteriocins could contribute to the ability of S. gallolyticus to colonize the colon where they have been associated with colorectal cancer.
dc.description.confidentialfalse
dc.edition.edition2020
dc.format.pagination13431-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/32778686
dc.identifier.citationHill D, O'Connor PM, Altermann E, Day L, Hill C, Stanton C, Ross RP. (2020). Extensive bacteriocin gene shuffling in the Streptococcus bovis/Streptococcus equinus complex reveals gallocin D with activity against vancomycin resistant enterococci.. Sci Rep. 10. 1. (pp. 13431-).
dc.identifier.doi10.1038/s41598-020-70328-z
dc.identifier.eissn2045-2322
dc.identifier.elements-typejournal-article
dc.identifier.issn2045-2322
dc.identifier.number13431
dc.identifier.pii10.1038/s41598-020-70328-z
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71352
dc.languageeng
dc.publisherSpringer Nature Limited
dc.publisher.urihttps://www.nature.com/articles/s41598-020-70328-z
dc.relation.isPartOfSci Rep
dc.rights(c) 2020 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAmino Acid Sequence
dc.subjectBacteriocins
dc.subjectComputer Simulation
dc.subjectDrug Resistance, Bacterial
dc.subjectSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
dc.subjectStreptococcal Infections
dc.subjectStreptococcus
dc.subjectStreptococcus bovis
dc.subjectVancomycin
dc.subjectVancomycin-Resistant Enterococci
dc.titleExtensive bacteriocin gene shuffling in the Streptococcus bovis/Streptococcus equinus complex reveals gallocin D with activity against vancomycin resistant enterococci.
dc.typeJournal article
pubs.elements-id486135
pubs.organisational-groupOther
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