Porcine colonoids and enteroids keep the memory of their origin during regeneration

Simple item page
dc.citation.issue5
dc.citation.volume320
dc.contributor.authorBarnett AM
dc.contributor.authorMullaney JA
dc.contributor.authorHendriks C
dc.contributor.authorLe Borgne L
dc.contributor.authorMcNabb WC
dc.contributor.authorRoy NC
dc.coverage.spatialUnited States
dc.date.accessioned2024-10-18T01:44:02Z
dc.date.available2024-10-18T01:44:02Z
dc.date.issued2021-05-01
dc.description.abstractThe development of alternative in vitro culture methods has increased in the last decade as three-dimensional organoids of various tissues, including those of the small and large intestines. Due to their multicellular composition, organoids offer advantages over traditionally used immortalized or primary cell lines. However, organoids must be accurate models of their tissues of origin. This study compared gene expression profiles with respect to markers of specific cell types (stem cells, enterocytes, goblet, and enteroendocrine cells) and barrier maturation (tight junctions) of colonoid and enteroid cultures with their tissues of origin and colonoids with enteroids. Colonoids derived from three healthy pigs formed multilobed structures with a monolayer of cells similar to the crypt structures in colonic tissue. Colonoid and enteroid gene expression signatures were more similar to those found for the tissues of their origin than to each other. However, relative to their derived tissues, organoids had increased gene expression levels of stem cell markers Sox9 and Lgr5 encoding sex-determining region Y-box 9 and leucine-rich repeat-containing G protein-coupled rector 5, respectively. In contrast, expression levels of Occl and Zo1 encoding occludin and zonula occludens 1, respectively, were decreased. Expression levels of the cell lineage markers Atoh1, Cga, and Muc2 encoding atonal homolog 1, chromogranin A, and mucin 2, respectively, were decreased in colonoids, whereas Sglt1 and Apn encoding sodium-glucose transporter 1 and aminopeptidase A, respectively, were decreased in enteroids. These results indicate colonoid and enteroid cultures were predominantly comprised of undifferentiated cell types with decreased barrier maturation relative to their tissues of origin.
dc.description.confidentialfalse
dc.edition.editionMay 2021
dc.format.paginationC794-C805
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/33760661
dc.identifier.citationBarnett AM, Mullaney JA, Hendriks C, Le Borgne L, McNabb WC, Roy NC. (2021). Porcine colonoids and enteroids keep the memory of their origin during regeneration.. Am J Physiol Cell Physiol. 320. 5. (pp. C794-C805).
dc.identifier.doi10.1152/ajpcell.00420.2020
dc.identifier.eissn1522-1563
dc.identifier.elements-typejournal-article
dc.identifier.issn0363-6143
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71762
dc.languageeng
dc.publisherAmerican Physiological Society
dc.publisher.urihttps://journals.physiology.org/doi/full/10.1152/ajpcell.00420.2020
dc.relation.isPartOfAm J Physiol Cell Physiol
dc.rights(c) 2021 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectbarrier maturation
dc.subjectcolon
dc.subjectdifferentiation
dc.subjectintestinal epithelium
dc.subjectstem cells
dc.subjectAnimals
dc.subjectBiomarkers
dc.subjectCell Differentiation
dc.subjectCell Lineage
dc.subjectCell Proliferation
dc.subjectColon
dc.subjectGene Expression Regulation
dc.subjectIleum
dc.subjectIntestinal Mucosa
dc.subjectMale
dc.subjectOrganoids
dc.subjectPhenotype
dc.subjectSignal Transduction
dc.subjectSus scrofa
dc.subjectTime Factors
dc.subjectTissue Culture Techniques
dc.subjectTranscriptome
dc.titlePorcine colonoids and enteroids keep the memory of their origin during regeneration
dc.typeJournal article
pubs.elements-id443322
pubs.organisational-groupOther
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