Branched pectic galactan in phloem-sieve-element cell walls: Implications for cell mechanics

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dc.citation.issue2
dc.citation.volume176
dc.contributor.authorTorode TA
dc.contributor.authorO'Neill R
dc.contributor.authorMarcus SE
dc.contributor.authorCornuault VRG
dc.contributor.authorPose S
dc.contributor.authorLauder RP
dc.contributor.authorKracun SK
dc.contributor.authorRydahl MG
dc.contributor.authorAndersen MCF
dc.contributor.authorWillats WGT
dc.contributor.authorBraybrook SA
dc.contributor.authorTownsend BJ
dc.contributor.authorClausen MH
dc.contributor.authorKnox JP
dc.date.available6/02/2018
dc.date.issued6/02/2018
dc.description.abstractA major question in plant biology concerns the specification and functional differentiation of cell types. This is in the context of constraints imposed by networks of cell walls that both adhere cells and contribute to the form and function of developing organs. Here, we report the identification of a glycan epitope that is specific to phloem sieve element cell walls in several systems. A monoclonal antibody, designated LM26, binds to the cell wall of phloem sieve elements in stems of Arabidopsis (Arabidopsis thaliana), Miscanthus x giganteus, and notably sugar beet (Beta vulgaris) roots where phloem identification is an important factor for the study of phloem unloading of Suc. Using microarrays of synthetic oligosaccharides, the LM26 epitope has been identified as a β-1,6-galactosyl substitution of β-1,4-galactan requiring more than three backbone residues for optimized recognition. This branched galactan structure has previously been identified in garlic (Allium sativum) bulbs in which the LM26 epitope is widespread throughout most cell walls including those of phloem cells. Garlic bulb cell wall material has been used to confirm the association of the LM26 epitope with cell wall pectic rhamnogalacturonan-I polysaccharides. In the phloem tissues of grass stems, the LM26 epitope has a complementary pattern to that of the LM5 linear β-1,4-galactan epitope, which is detected only in companion cell walls. Mechanical probing of transverse sections of M x giganteus stems and leaves by atomic force microscopy indicates that phloem sieve element cell walls have a lower indentation modulus (indicative of higher elasticity) than companion cell walls.
dc.description.confidentialFALSE
dc.format.extent1547 - 1558
dc.identifier.citationPlant physiology, 2018, 176 (2), pp. 1547 - 1558
dc.identifier.doi10.1104/pp.17.01568
dc.identifier.elements-id403207
dc.identifier.harvestedMassey_Dark
dc.identifier.issn0032-0889
dc.identifier.urihttps://hdl.handle.net/10179/13067
dc.languageEnglish
dc.publisherAmerican Society of Plant Biologists
dc.relation.isPartOfPlant physiology
dc.relation.urihttp://www.plantphysiol.org/content/176/2/1547.full
dc.subject.anzsrc06 Biological Sciences
dc.subject.anzsrc07 Agricultural and Veterinary Sciences
dc.titleBranched pectic galactan in phloem-sieve-element cell walls: Implications for cell mechanics
dc.typeJournal article
pubs.notesNot known
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Sciences
pubs.organisational-group/Massey University/College of Sciences/Institute of Fundamental Sciences
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